Summary of Gfi-1 rescue
| . | Gfi-1 mRNA (%) . | CD11b+Gr1+ (%) . | CD11b GeoMean . | F4/80+Mac3+ (%) . |
|---|---|---|---|---|
| Control: MSCV-puro | 100 | 40.0 + 2.8 | 10 200 + 6800 | 4.1 + 3.3 |
| Control: MSCV-Gfi1 | 161 + 27 | 36.0 + 6.0 | 8900 + 3100 | 4.4 + 4.1 |
| KO: MSCV-puro | 34 + 5* | 16.5 + 2.7* | 2300 + 1500* | 25.9 + 6.0* |
| KO: MSCV-Gfi1 | 115 + 40 | 26.4 + 1.9 | 4700 + 2200 | 8.1 + 7.0 |
| . | Gfi-1 mRNA (%) . | CD11b+Gr1+ (%) . | CD11b GeoMean . | F4/80+Mac3+ (%) . |
|---|---|---|---|---|
| Control: MSCV-puro | 100 | 40.0 + 2.8 | 10 200 + 6800 | 4.1 + 3.3 |
| Control: MSCV-Gfi1 | 161 + 27 | 36.0 + 6.0 | 8900 + 3100 | 4.4 + 4.1 |
| KO: MSCV-puro | 34 + 5* | 16.5 + 2.7* | 2300 + 1500* | 25.9 + 6.0* |
| KO: MSCV-Gfi1 | 115 + 40 | 26.4 + 1.9 | 4700 + 2200 | 8.1 + 7.0 |
Summary of rescue of KO bone marrow cells in 4 distinct experiments, with MSCV–Gfi-1 or MSCV empty vector, subjected to flow cytometry for CD11b, Gr1, F4/80, and Mac3, presented as percentage of positive cells and geometric mean fluorescence.
P < 0.05 by ANOVA.