Physicochemical properties of anti-CLEC9A– and RbIgG-coated PLGA nanoparticles
| Samples . | Nanoparticles diameter, mn, ± SD . | Polydispersity index, ± SD . | Zeta potential, mV, ± SD . | gp100, μg/mg PLGA . | Antibodies, μg/mg PLGA . |
|---|---|---|---|---|---|
| αCLEC9A-NP | 243.4 ± 7.2 | 0.210 ± 0.048 | −16.4 ± 1.7 | 30 | 20 |
| RbIgG-NP | 247.5 ± 9.8 | 0.259 ± 0.029 | −17.2 ± 1.6 | 30 | 22 |
| Samples . | Nanoparticles diameter, mn, ± SD . | Polydispersity index, ± SD . | Zeta potential, mV, ± SD . | gp100, μg/mg PLGA . | Antibodies, μg/mg PLGA . |
|---|---|---|---|---|---|
| αCLEC9A-NP | 243.4 ± 7.2 | 0.210 ± 0.048 | −16.4 ± 1.7 | 30 | 20 |
| RbIgG-NP | 247.5 ± 9.8 | 0.259 ± 0.029 | −17.2 ± 1.6 | 30 | 22 |
PLGA nanoparticles with gp100:272-300 long peptide and Atto488 coated with αCLEC9A or RbIgG via protein A were characterized by DLS and ζ potential measurements. Nanoparticles diameter data represent the mean value ± SD and the polydispersity index as measured by DLS. Zeta potential data represent the mean value ± SD of five readings. Loading efficiency of gp100:272-300 long peptide was measured by Coomassie dye protein assay. The conjugation efficiency of αCLEC9A and RbIgG was determined by Coomassie dye protein assay.
PLGA indicates poly(lactic-co-glycolic acid).