Predicted peptides derived from candidate Ags DAPK2 and PIM1
| Protein . | ID . | Position . | Sequence . | Consensus rank* . | T2 binding . | |
|---|---|---|---|---|---|---|
| FI-0 . | FI-24 . | |||||
| Influenza M1 | M1 | 58 | GILGFVFTL | n/a | 2.34 | 2.17 |
| DAPK2 | D1 | 156 | MLLDKNIPI | 3 | 3.92 | 2.28 |
| D2 | 316 | KLSFSIVSL | 6 | 0.69 | 0.06 | |
| D3 | 220 | LLSGASPFL | 10 | 1.78 | 0.98 | |
| D4 | 129 | FIKQILDGV | 13 | 0.13 | 0 | |
| D5 | 132 | QILDGVNYL | 17 | 1.92 | 0.18 | |
| D6 | 205 | GLEADMWSI | 19 | 1.50 | 0 | |
| D7 | 186 | NIFGTPEFV | 26 | 2.99 | 0.07 | |
| D8 | 81 | VLHHNVITL | 26 | 2.05 | 0.36 | |
| D9 | 211 | WSIGVITYI | 29 | 0.14 | 0 | |
| D10 | 212 | SIGVITYIL | 35 | 0.23 | 0 | |
| PIM1 (isoform 2) | P1 | 1 | MLLSKINSL | 3 | 1.19 | 0 |
| P2 | 183 | KLIDFGSGA | 7 | 0.17 | 0 | |
| P3 | 118 | LILERPEPV | 14 | 1.56 | 2.68 | |
| P4 | 191 | ALLKDTVYT | 16 | 1.03 | 0 | |
| P5 | 147 | FFWQVLEAV | 16 | 0.57 | 0.04 | |
| P6 | 265 | HLIRWCLAL | 21 | 0.85 | 0.14 | |
| P7 | 2 | LLSKINSLA | 21 | 1.8 | 0.39 | |
| Protein . | ID . | Position . | Sequence . | Consensus rank* . | T2 binding . | |
|---|---|---|---|---|---|---|
| FI-0 . | FI-24 . | |||||
| Influenza M1 | M1 | 58 | GILGFVFTL | n/a | 2.34 | 2.17 |
| DAPK2 | D1 | 156 | MLLDKNIPI | 3 | 3.92 | 2.28 |
| D2 | 316 | KLSFSIVSL | 6 | 0.69 | 0.06 | |
| D3 | 220 | LLSGASPFL | 10 | 1.78 | 0.98 | |
| D4 | 129 | FIKQILDGV | 13 | 0.13 | 0 | |
| D5 | 132 | QILDGVNYL | 17 | 1.92 | 0.18 | |
| D6 | 205 | GLEADMWSI | 19 | 1.50 | 0 | |
| D7 | 186 | NIFGTPEFV | 26 | 2.99 | 0.07 | |
| D8 | 81 | VLHHNVITL | 26 | 2.05 | 0.36 | |
| D9 | 211 | WSIGVITYI | 29 | 0.14 | 0 | |
| D10 | 212 | SIGVITYIL | 35 | 0.23 | 0 | |
| PIM1 (isoform 2) | P1 | 1 | MLLSKINSL | 3 | 1.19 | 0 |
| P2 | 183 | KLIDFGSGA | 7 | 0.17 | 0 | |
| P3 | 118 | LILERPEPV | 14 | 1.56 | 2.68 | |
| P4 | 191 | ALLKDTVYT | 16 | 1.03 | 0 | |
| P5 | 147 | FFWQVLEAV | 16 | 0.57 | 0.04 | |
| P6 | 265 | HLIRWCLAL | 21 | 0.85 | 0.14 | |
| P7 | 2 | LLSKINSLA | 21 | 1.8 | 0.39 | |
Consensus rank score represents the composite ranking of peptides predicted by all the 3 servers (IEDB-ANN, NetMHC-ANN, and MHC-I multiple matrix). The lower the composite rank number, the higher the predicted binding affinity.
FI-0 indicates the fluorescence intensity at 0 hours; and FI-24, fluorescence intensity at 24 hours.