Primer sequences
| . | Sequences . |
|---|---|
| ddx18hi1727 genotyping | ddx18 forward: 5′-GCGGACATGCAGATGAAGAT-3′ |
| Reverse viral (nltr3): 5′-CTGTTCCATCTGTTCCTGAC-3′ | |
| ddx18 reverse wt: 5′-CATTAAATCACACATAGCCACGA-3′ | |
| Zebrafish ddx18 short probe | Forward: 5′-ggctattcatgggaagcaga-3′ |
| Reverse: 5′-TGTAGGCCTCCTGAGCTGAT-3′ | |
| Zebrafish ddx18 qPCR | Forward: 5′-CGGCTGAGGAGGATAGTGATG-3′ |
| Reverse: 5′-ACGCACCTGTCAGACCAGAAG-3′ | |
| Zebrafish β-actin qPCR | Forward: 5′-CTGGTCGTTGACAACGGCT-3′ |
| Reverse: 5′-TCCATCACAATACCAGTAGT-3′ | |
| Human DDX18 qPCR | Forward: 5′-GGGACTGACAGGAGCTTTTGAG-3′ |
| Reverse: 5′-GCCTTCAGAGTGTTTTCATTGACA-3′ | |
| Site-directed mutagenesis primers | hsDDX18_G548A: 5′-GGATACTTCGTTTGCTTCTCTATATAATCTTGTCAATGAAAACACTC-3′ |
| hsDDX18_G548A-r: 5′-GAGTGTTTTCATTGACAAGATTATATAGAGAAGCAAACGAAGTATCC-3′ | |
| hsDDX18_G1111A: 5′-GCCACCCAAACTCGAAAAATTGAAGACCTGGCAA-3′ | |
| hsDDX18_G1111A-r: 5′-TTGCCAGGTCTTCAATTTTTCGAGTTTGGGTGGC-3′ | |
| hsDDX18_G1861A: 5′-TCAAGGTGCCTCCCTTCATTGATCTGAACGTCAAC-3′ | |
| hsDDX18_G1861A-r: 5′-GTTGACGTTCAGATCAATGAAGGGAGGCACCTTGA-3′ | |
| Human DDX18 sequencing primers | Forward: 5′-CGGGATCCTGTTGGGCAGAATGTCACAC-3′ |
| Reverse: 5′-ACTCGAGAAGGAAGGCATGTGTTCAGTG-3′ | |
| middle: 5′-TCCTCTTCAAACCCCACATC-3′ | |
| middle2: 5′-GCTTGATAATGGGTGGCAGT-3′ |
| . | Sequences . |
|---|---|
| ddx18hi1727 genotyping | ddx18 forward: 5′-GCGGACATGCAGATGAAGAT-3′ |
| Reverse viral (nltr3): 5′-CTGTTCCATCTGTTCCTGAC-3′ | |
| ddx18 reverse wt: 5′-CATTAAATCACACATAGCCACGA-3′ | |
| Zebrafish ddx18 short probe | Forward: 5′-ggctattcatgggaagcaga-3′ |
| Reverse: 5′-TGTAGGCCTCCTGAGCTGAT-3′ | |
| Zebrafish ddx18 qPCR | Forward: 5′-CGGCTGAGGAGGATAGTGATG-3′ |
| Reverse: 5′-ACGCACCTGTCAGACCAGAAG-3′ | |
| Zebrafish β-actin qPCR | Forward: 5′-CTGGTCGTTGACAACGGCT-3′ |
| Reverse: 5′-TCCATCACAATACCAGTAGT-3′ | |
| Human DDX18 qPCR | Forward: 5′-GGGACTGACAGGAGCTTTTGAG-3′ |
| Reverse: 5′-GCCTTCAGAGTGTTTTCATTGACA-3′ | |
| Site-directed mutagenesis primers | hsDDX18_G548A: 5′-GGATACTTCGTTTGCTTCTCTATATAATCTTGTCAATGAAAACACTC-3′ |
| hsDDX18_G548A-r: 5′-GAGTGTTTTCATTGACAAGATTATATAGAGAAGCAAACGAAGTATCC-3′ | |
| hsDDX18_G1111A: 5′-GCCACCCAAACTCGAAAAATTGAAGACCTGGCAA-3′ | |
| hsDDX18_G1111A-r: 5′-TTGCCAGGTCTTCAATTTTTCGAGTTTGGGTGGC-3′ | |
| hsDDX18_G1861A: 5′-TCAAGGTGCCTCCCTTCATTGATCTGAACGTCAAC-3′ | |
| hsDDX18_G1861A-r: 5′-GTTGACGTTCAGATCAATGAAGGGAGGCACCTTGA-3′ | |
| Human DDX18 sequencing primers | Forward: 5′-CGGGATCCTGTTGGGCAGAATGTCACAC-3′ |
| Reverse: 5′-ACTCGAGAAGGAAGGCATGTGTTCAGTG-3′ | |
| middle: 5′-TCCTCTTCAAACCCCACATC-3′ | |
| middle2: 5′-GCTTGATAATGGGTGGCAGT-3′ |
Bold, underlined text indicates restriction enzyme linkers.