Table 2 Affinity of His-tagged CC2-LZ WT... | American Society of Hematology

Table 2

Affinity of His-tagged CC2-LZ WT and of the D311N and D311G mutants for linear and K63-linked tetraubiquitin chains

	Tetraubiquitin chains (K_d in μM)		^mutK_d/^WTK_d
	K63	Linear	K63	Linear
WT	4 ± 1	0.9 ± 0.3	1	1
D311N	56 ± 16	14 ± 4	14	15.6
D311G	112 ± 30	29 ± 9	28	32

	Tetraubiquitin chains (K_d in μM)		^mutK_d/^WTK_d
	K63	Linear	K63	Linear
WT	4 ± 1	0.9 ± 0.3	1	1
D311N	56 ± 16	14 ± 4	14	15.6
D311G	112 ± 30	29 ± 9	28	32

^mutK_d/^WTK_d indicates the difference in affinity with respect to the WT for linear and K63-linked tetraubiquitin chains.

Binding constants were calculated by densitometry, as described in “Methods”, in the pull-down experiments shown in Figure 2.