Hematological and biochemical parameters of peripheral blood
| Genotype . | RbLoxP/LoxP . | RbΔ/Δ . | E2f8Δ/Δ . | RbΔ/Δ E2f8Δ/Δ . | RbΔ/Δ E2f7Δ/Δ E2f8Δ/Δ . | RbΔ/Δ E2f7Δ/Δ . | E2f7Δ/Δ E2f8Δ/Δ . |
|---|---|---|---|---|---|---|---|
| RBCs (× 109/L) | 9.2 ± 0.3 | 6.9 ± 0.3*** | 8.6 ± 0.4** | 4.3 ± 0.2*** (***) | 4.1 ± 0.7*** (***) | 7.6 ± 0.3*** (**) | 8.6 ± 0.7* |
| Hemoglobin (g/dL) | 14.7 ± 0.8 | 11.9 ± 0.6*** | 14.4 ± 0.4 | 8.0 ± 0.2*** (***) | 8.6 ± 0.7*** (***) | 13.0 ± 0.8** (*) | 14.4 ± 0.4 |
| Hematocrit (%) | 41.4 ± 1.8 | 33.3 ± 2.4*** | 39.4 ± 3.5 | 24.5 ± 1.2*** (***) | 23.7 ± 3.3*** (***) | 37.9 ± 2.5** (**) | 38.7 ± 2.6* |
| MCV(fL) | 44.8 ± 1.7 | 48.8 ± 2.9** | 44.5 ± 1.0 | 57.6 ± 2.4*** (***) | 58.0 ± 2.7*** (***) | 50.1 ± 1.8*** | 45.0 ± 1.3 |
| MCH(fg) | 15.9 ± 1.0 | 17.4 ± 0.8** | 15.0 ± 0.8 | 18.7 ± 0.6*** (**) | 21.2 ± 2.2*** (***) | 17.2 ± 0.4* | 16.7 ± 0.9 |
| MCHC (g/dL) | 35.5 ± 1.3 | 35.7 ± 2.4 | 35.0 ± 2.3 | 32.6 ± 1.3** (*) | 36.5 ± 2.6 | 34.3 ± 1.1 | 37.1 ± 1.4* |
| RDW (%) | 17.7 ± 2.5 | 21.3 ± 2.2** | 17.2 ± 1.1 | 25.3 ± 3.2*** (*) | 27.1 ± 1.3*** (**) | ND | ND |
| Reticulocyte (× 108/L) | 2.1 ± 0.2 | 3.2 ± 0.6* | 2.6 ± 0.4 | 9.5 ± 1.5*** (**) | 8.8 ± 0.6*** (***) | ND | ND |
| nRBC (per 5000 RBCs) | 0 ± 0 | 0 ± 0 | 0 ± 0 | 4.4 ± 0.9** (**) | ND | ND | ND |
| Erythropoietin (pg/mL) | 185.4 ± 16 | 468.6 ± 7*** | ND | 1944.2 ± 143*** (***) | 1986.0 ± 173*** (***) | ND | ND |
| Total bilirubin (mg/dL) | 0.4 ± 0 | 0.3 ± 0 | ND | 0.8 ± 0.1 | 1.2 ± 0.2 | ND | ND |
| Unconjugated bilirubin (mg/dL) | 0.3 ± 0 | 0.3 ± 0 | ND | 0.7 ± 0.1 | 1.0 ± 0.1 | ND | ND |
| Genotype . | RbLoxP/LoxP . | RbΔ/Δ . | E2f8Δ/Δ . | RbΔ/Δ E2f8Δ/Δ . | RbΔ/Δ E2f7Δ/Δ E2f8Δ/Δ . | RbΔ/Δ E2f7Δ/Δ . | E2f7Δ/Δ E2f8Δ/Δ . |
|---|---|---|---|---|---|---|---|
| RBCs (× 109/L) | 9.2 ± 0.3 | 6.9 ± 0.3*** | 8.6 ± 0.4** | 4.3 ± 0.2*** (***) | 4.1 ± 0.7*** (***) | 7.6 ± 0.3*** (**) | 8.6 ± 0.7* |
| Hemoglobin (g/dL) | 14.7 ± 0.8 | 11.9 ± 0.6*** | 14.4 ± 0.4 | 8.0 ± 0.2*** (***) | 8.6 ± 0.7*** (***) | 13.0 ± 0.8** (*) | 14.4 ± 0.4 |
| Hematocrit (%) | 41.4 ± 1.8 | 33.3 ± 2.4*** | 39.4 ± 3.5 | 24.5 ± 1.2*** (***) | 23.7 ± 3.3*** (***) | 37.9 ± 2.5** (**) | 38.7 ± 2.6* |
| MCV(fL) | 44.8 ± 1.7 | 48.8 ± 2.9** | 44.5 ± 1.0 | 57.6 ± 2.4*** (***) | 58.0 ± 2.7*** (***) | 50.1 ± 1.8*** | 45.0 ± 1.3 |
| MCH(fg) | 15.9 ± 1.0 | 17.4 ± 0.8** | 15.0 ± 0.8 | 18.7 ± 0.6*** (**) | 21.2 ± 2.2*** (***) | 17.2 ± 0.4* | 16.7 ± 0.9 |
| MCHC (g/dL) | 35.5 ± 1.3 | 35.7 ± 2.4 | 35.0 ± 2.3 | 32.6 ± 1.3** (*) | 36.5 ± 2.6 | 34.3 ± 1.1 | 37.1 ± 1.4* |
| RDW (%) | 17.7 ± 2.5 | 21.3 ± 2.2** | 17.2 ± 1.1 | 25.3 ± 3.2*** (*) | 27.1 ± 1.3*** (**) | ND | ND |
| Reticulocyte (× 108/L) | 2.1 ± 0.2 | 3.2 ± 0.6* | 2.6 ± 0.4 | 9.5 ± 1.5*** (**) | 8.8 ± 0.6*** (***) | ND | ND |
| nRBC (per 5000 RBCs) | 0 ± 0 | 0 ± 0 | 0 ± 0 | 4.4 ± 0.9** (**) | ND | ND | ND |
| Erythropoietin (pg/mL) | 185.4 ± 16 | 468.6 ± 7*** | ND | 1944.2 ± 143*** (***) | 1986.0 ± 173*** (***) | ND | ND |
| Total bilirubin (mg/dL) | 0.4 ± 0 | 0.3 ± 0 | ND | 0.8 ± 0.1 | 1.2 ± 0.2 | ND | ND |
| Unconjugated bilirubin (mg/dL) | 0.3 ± 0 | 0.3 ± 0 | ND | 0.7 ± 0.1 | 1.0 ± 0.1 | ND | ND |
Shown are mean values ± SD. n ≥ 3 per genotypic groups except for total bilirubin and unconjugated bilirubin assays (n = 2). Asterisks represent statistical comparisons to RbLoxP/LoxP mice, whereas asterisks in parentheses represent statistical comparisons to RbΔ/Δ mice.
nRBC indicates nucleated RBC; RDW, red cell distribution width; and ND, not determined.
P < .05.
P < .01.
P < .001.