Table 1 Reprogramming of human Fanconi... | American Society of Hematology

Table 1

Reprogramming of human Fanconi anemia cells is impaired

Sample no.	Complementation group*	Vector	Efficiency % (no. iPSC clones)	Oxygen tension†
1	Normal donor	Lenti	0.01 (12)	5
2	FA-A	Lenti	0.001 (2)	5
2	FA-A + FANCA	Lenti	0.003 (6)	5
3	FA-A	Lenti	0	5
3	FA-A + FANCA	Lenti	0.003 (3)	5
4	FA-C	Lenti	0.001(1)	5
4	FA-C + FANCC	Lenti	0.002 (2)	5
5-7	FA-A	Retro	0	21
8,9	FA-C	Retro	0	21
10	FA-G	Retro	0	21
11	FA-D2	Retro	0	21

Sample no.	Complementation group*	Vector	Efficiency % (no. iPSC clones)	Oxygen tension†
1	Normal donor	Lenti	0.01 (12)	5
2	FA-A	Lenti	0.001 (2)	5
2	FA-A + FANCA	Lenti	0.003 (6)	5
3	FA-A	Lenti	0	5
3	FA-A + FANCA	Lenti	0.003 (3)	5
4	FA-C	Lenti	0.001(1)	5
4	FA-C + FANCC	Lenti	0.002 (2)	5
5-7	FA-A	Retro	0	21
8,9	FA-C	Retro	0	21
10	FA-G	Retro	0	21
11	FA-D2	Retro	0	21

Fibroblasts (1 × 10⁵) were reprogrammed. The number of independent reprogramming experiments, type of reprogramming vector used, and oxygen tension are shown. Efficiency is expressed as the number of iPSC clones derived per number of input fibroblasts.

Plus or minus genetic correction.

†

Precent O₂.

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