Comparative immunogenicity of wt fVIII, R372A/R1689A fVIII, and R372A/R1689A fVIII in fVIII−/− mice
| fVIII construct . | Dose, μg . | No. of ELISA titer positive . | Mean/median ELISA titer . | No. of inhibitor titer positive . | Mean/median inhibitor titer, BU/mL . |
|---|---|---|---|---|---|
| wt fVIII | 0.2* | 17/25 | 1490/400 | 13/25 | 44/10 |
| 0.5 | 2/9 | 9/0 | 1/9 | 1/0 | |
| 1.0 | 5/9 | 100/11 | 3/9 | 14/0 | |
| 1.5 | 9/9 | 460/426 | 9/9 | 100/73 | |
| 2.0 | 10/10 | 1540/1760 | 10/10 | 310/310 | |
| R372A/R1689A fVIII | 0.2* | 10/25 | 470/0 | 6/25 | 4/0 |
| 0.5 | 1/9 | 120/0 | 1/9 | 8/0 | |
| 1.0 | 5/9 | 170/16 | 3/9 | 29/0 | |
| 1.5 | 6/9 | 1170/74 | 6/9 | 160/8 | |
| 2.0 | 10/10 | 730/450 | 10/10 | 160/103 | |
| V634M fVIII | 0.5 | 3/9 | 190/0 | 1/9 | 11/0 |
| 1.0 | 5/9 | 140/10 | 4/9 | 16/0 | |
| 1.5 | 6/9 | 630/560 | 6/9 | 130/88 | |
| 2.0 | 9/10 | 2810/1480 | 9/10 | 450/290 |
| fVIII construct . | Dose, μg . | No. of ELISA titer positive . | Mean/median ELISA titer . | No. of inhibitor titer positive . | Mean/median inhibitor titer, BU/mL . |
|---|---|---|---|---|---|
| wt fVIII | 0.2* | 17/25 | 1490/400 | 13/25 | 44/10 |
| 0.5 | 2/9 | 9/0 | 1/9 | 1/0 | |
| 1.0 | 5/9 | 100/11 | 3/9 | 14/0 | |
| 1.5 | 9/9 | 460/426 | 9/9 | 100/73 | |
| 2.0 | 10/10 | 1540/1760 | 10/10 | 310/310 | |
| R372A/R1689A fVIII | 0.2* | 10/25 | 470/0 | 6/25 | 4/0 |
| 0.5 | 1/9 | 120/0 | 1/9 | 8/0 | |
| 1.0 | 5/9 | 170/16 | 3/9 | 29/0 | |
| 1.5 | 6/9 | 1170/74 | 6/9 | 160/8 | |
| 2.0 | 10/10 | 730/450 | 10/10 | 160/103 | |
| V634M fVIII | 0.5 | 3/9 | 190/0 | 1/9 | 11/0 |
| 1.0 | 5/9 | 140/10 | 4/9 | 16/0 | |
| 1.5 | 6/9 | 630/560 | 6/9 | 130/88 | |
| 2.0 | 9/10 | 2810/1480 | 9/10 | 450/290 |
P < .05 when compared with wt fVIII.