Coordinate induction of dleu2-miR-15a-16 after HDAC inhibition
| Sample . | 13q deletion . | miR-15a . | miR-16 . | Dleu2 . | Smc4 . |
|---|---|---|---|---|---|
| 1 | 98% | 2.4 | 2.9 | 3.8 | 1 |
| 2 | 49% | 1.4 | 1.9 | 2.1 | 1.1 |
| 3 | 40% | - | 1.6 | 2 | 0.7 |
| 4 | 40% | 6 | 6.1 | ND | 0.5 |
| 5 | 15% | 2 | 1.7 | 1.8 | 0.1 |
| 6 | 13% | 1.7 | 1.7 | 5 | 0.3 |
| 7 | 0% | 1.8 | 2.2 | 2.4 | 0.6 |
| 8 | 0% | 1.4 | 1.4 | 2 | ND |
| 9* | 0% | 3 | 3 | 3.1 | ND |
| 9† | 0% | 5.1 | 4.3 | 4.1 | ND |
| 10 | 91% | - | 5.3 | ND | 2 |
| 11 | Not done | 5.8 | 3.2 | ND | 3 |
| Sample . | 13q deletion . | miR-15a . | miR-16 . | Dleu2 . | Smc4 . |
|---|---|---|---|---|---|
| 1 | 98% | 2.4 | 2.9 | 3.8 | 1 |
| 2 | 49% | 1.4 | 1.9 | 2.1 | 1.1 |
| 3 | 40% | - | 1.6 | 2 | 0.7 |
| 4 | 40% | 6 | 6.1 | ND | 0.5 |
| 5 | 15% | 2 | 1.7 | 1.8 | 0.1 |
| 6 | 13% | 1.7 | 1.7 | 5 | 0.3 |
| 7 | 0% | 1.8 | 2.2 | 2.4 | 0.6 |
| 8 | 0% | 1.4 | 1.4 | 2 | ND |
| 9* | 0% | 3 | 3 | 3.1 | ND |
| 9† | 0% | 5.1 | 4.3 | 4.1 | ND |
| 10 | 91% | - | 5.3 | ND | 2 |
| 11 | Not done | 5.8 | 3.2 | ND | 3 |
A cohort of 12 CLL samples that demonstrated increases in miR-15a and miR-16 upon HDAC inhibition was evaluated for expression levels of the host genes dleu2 and Smc4 before and after exposure to LBH589, SAHA, or MS275 for 3-6 hours. Data represent the -fold increase in expression over untreated cells.
ND indicates not detectable.
Sample exposed to 3mM MS275 for 3 hours.
Sample exposed to 3mM SAHA for 3 hours.