Genotyping of pre-B, GM, and CFUe colonies by PCR shows that no NF-Ya−/− colonies were obtained
| . | +/+ +Mx cre . | fl/fl +Mx cre . | |||||
|---|---|---|---|---|---|---|---|
| +/+ . | fl/+ . | −/− . | +/+ . | fl/fl . | fl/− . | −/− . | |
| Pre-B | 65 | 0 | 0 | 2 | 36 | 29 | 0 |
| GM | 87 | 0 | 0 | 5 | 52 | 47 | 0 |
| CFUe | 57 | 0 | 0 | 1 | 42 | 21 | 0 |
| . | +/+ +Mx cre . | fl/fl +Mx cre . | |||||
|---|---|---|---|---|---|---|---|
| +/+ . | fl/+ . | −/− . | +/+ . | fl/fl . | fl/− . | −/− . | |
| Pre-B | 65 | 0 | 0 | 2 | 36 | 29 | 0 |
| GM | 87 | 0 | 0 | 5 | 52 | 47 | 0 |
| CFUe | 57 | 0 | 0 | 1 | 42 | 21 | 0 |
B cell, myeloid, and erythroid precursors of BM cells of NF-Ya+/++Mx cre (control) and NF-Yafl/fl+Mx cre (ko) BM chimera were grown in methylcellulose. The resulting colonies were randomly picked and genotyped.