Expression levels of cell cycle– and apoptosis-related genes are dysregulated in NF-Ya ko BM cells
| . | Day 1 HSCs . | Day 1 total BM . | Day 3 total BM . | |||
|---|---|---|---|---|---|---|
| Average ± SD . | P . | Average ± SD . | P . | Average ± SD . | P . | |
| Ccnb1 | 92.6 ± 28.4 | .29 | 92.3 ± 10.5 | .08 | 48.3 ± 6.4 | < .01 |
| Cdkn1α | 63.5 ± 17.4 | < .01 | 40.0 ± 34.0 | < .01 | 29.3 ± 20.0 | < .01 |
| Bax | 55.3 ± 14.2 | < .01 | 41.6 ± 29.1 | < .01 | 66.3 ± 17.1 | .04 |
| Bcl-2 | 62.8 ± 25.9 | .02 | 54.7 ± 28.6 | .05 | 22.5 ± 5.9 | < .01 |
| Bax/Bcl-2 | 0.9 ± 0.6 | 0.8 ± 0.8 | 3.0 ± 1.5 | |||
| . | Day 1 HSCs . | Day 1 total BM . | Day 3 total BM . | |||
|---|---|---|---|---|---|---|
| Average ± SD . | P . | Average ± SD . | P . | Average ± SD . | P . | |
| Ccnb1 | 92.6 ± 28.4 | .29 | 92.3 ± 10.5 | .08 | 48.3 ± 6.4 | < .01 |
| Cdkn1α | 63.5 ± 17.4 | < .01 | 40.0 ± 34.0 | < .01 | 29.3 ± 20.0 | < .01 |
| Bax | 55.3 ± 14.2 | < .01 | 41.6 ± 29.1 | < .01 | 66.3 ± 17.1 | .04 |
| Bcl-2 | 62.8 ± 25.9 | .02 | 54.7 ± 28.6 | .05 | 22.5 ± 5.9 | < .01 |
| Bax/Bcl-2 | 0.9 ± 0.6 | 0.8 ± 0.8 | 3.0 ± 1.5 | |||
RNA was isolated from BM cells 1 or 3 days after pIpC treatment. Equal amounts of RNA were reverse transcribed and subsequently subjected to quantitative PCR. The table shows average ± SD expression levels of the indicated genes in ko samples in percentage of control samples (n ≥ 4).