Platelet activation flow cytometry parameters
| . | Resting . | ADP activated . | ||||||
|---|---|---|---|---|---|---|---|---|
| WT . | MKL1 KO . | MKL2 cKO . | DKO . | WT . | MKL1 KO . | MKL2 cKO . | DKO . | |
| Mean FSC | 28 ± .32 | 25 ± .84 | 29 ± .42 | 47 ± 3.9* | 30 ± .53 | 29 ± .46 | 33 ± 1.2 | 52 ± 4.9* |
| JON/A, MFI | 17 ± .4 | 16 ± .26 | 17 ± .25 | 20 ± 1.0* | 106 ± 16 | 104 ± 11 | 108 ± 48 | 30 ± 1.4* |
| . | Resting . | ADP activated . | ||||||
|---|---|---|---|---|---|---|---|---|
| WT . | MKL1 KO . | MKL2 cKO . | DKO . | WT . | MKL1 KO . | MKL2 cKO . | DKO . | |
| Mean FSC | 28 ± .32 | 25 ± .84 | 29 ± .42 | 47 ± 3.9* | 30 ± .53 | 29 ± .46 | 33 ± 1.2 | 52 ± 4.9* |
| JON/A, MFI | 17 ± .4 | 16 ± .26 | 17 ± .25 | 20 ± 1.0* | 106 ± 16 | 104 ± 11 | 108 ± 48 | 30 ± 1.4* |
Mean FSC and MFI of JON/A staining was averaged and the SD displayed (n = 4).
ADP indicates adenosine 5′-diphosphate; WT, wild type; KO, knockout; cKO, conditional knockout; DKO, double knockout; FSC, forward scatter; and MFI, mean fluorescence intensity.
P < .001.