DKO and Srf Pf4-cKO megakaryocytes have differential megakaryocyte transmembrane gene expression
| Gene . | FPKM . | ||||
|---|---|---|---|---|---|
| WT . | SRF cKO . | MKL1 KO . | MKL2 cKO . | DKO . | |
| 1110008F13Rik | 168.39 | 108.66 | 124.53 | 118.02 | 162.05 |
| Alox5ap | 2324.05 | 1609.63 | 2435.91 | 2216.10 | 2811.82 |
| Atp5g3 | 385.29 | 244.15 | 250.03 | 242.72 | 508.98 |
| Bsg | 319.57 | 233.30 | 278.88 | 310.46 | 513.28 |
| Cd151 | 87.72 | 52.09 | 79.72 | 89.09 | 33.04* |
| Cd47 | 30.43 | 31.40 | 25.75 | 23.65 | 0.00 |
| Cd63 | 555.94 | 402.13 | 499.11 | 485.78 | 921.27 |
| Cd9 | 1630.72 | 852.78 | 1449.95 | 1627.59 | 619.83 |
| Cox4i1 | 521.67 | 401.89 | 414.34 | 408.12 | 1190.58 |
| Cox8a | 238.02 | 173.46 | 161.16 | 176.70 | 428.98 |
| Cyba | 155.52 | 323.36 | 134.54 | 87.42 | 765.77* |
| Dhcr24 | 148.52 | 76.21 | 124.12 | 133.68 | 32.72* |
| Dnajb4 | 42.37 | 25.26 | 36.92 | 47.77 | 11.62* |
| Esam | 126.13 | 68.79 | 80.62 | 146.31 | 91.92 |
| Eya2 | 31.29 | 14.01* | 41.15 | 24.88 | 10.51* |
| Fads2 | 207.86 | 130.48 | 172.31 | 254.37 | 50.26* |
| Fcer1g | 542.87 | 519.26 | 403.81 | 409.80 | 682.78 |
| Glipr1 | 245.40 | 205.21 | 238.57 | 161.53 | 227.67 |
| Gp1ba | 225.28 | 101.01* | 198.97 | 304.22 | 20.66* |
| Gp1bb | 68.88 | 31.31 | 47.20 | 51.64 | 73.57 |
| Gp49a | 812.14 | 447.61 | 810.08 | 724.34 | 192.64* |
| Gp5 | 270.36 | 99.97* | 183.32 | 242.73 | 103.53 |
| Gp9 | 307.39 | 118.95* | 222.33 | 264.54 | 212.19 |
| Gpr56 | 855.30 | 417.16 | 772.67 | 979.05 | 367.96 |
| H2-K1 | 602.78 | 843.12 | 527.17 | 490.93 | 1433.99* |
| Ifitm1 | 318.43 | 319.23 | 317.53 | 247.40 | 1536.24* |
| Ifitm2 | 263.50 | 278.33 | 236.67 | 250.93 | 501.76 |
| Ifitm3 | 580.84 | 578.90 | 410.03 | 463.05 | 1026.08 |
| Itga2b | 2506.71 | 1231.66* | 2305.33 | 2301.65 | 1637.24 |
| Itga6 | 665.79 | 277.35* | 625.44 | 736.21 | 50.74* |
| Itm2b | 691.15 | 965.94 | 734.92 | 600.35 | 739.78 |
| Laptm4a | 138.76 | 107.84 | 125.00 | 133.70 | 102.47 |
| Laptm5 | 2043.75 | 1786.38 | 2362.76 | 2407.72 | 512.81 |
| Lilrb4 | 1168.14 | 626.80 | 1169.76 | 1092.51 | 273.49* |
| Mcl1 | 112.29 | 129.28 | 127.35 | 88.38 | 57.66 |
| Mpl | 92.44 | 40.82* | 57.96 | 116.22 | 23.74* |
| Nrgn | 437.18 | 203.67 | 256.78 | 303.27 | 226.77 |
| P2rx1 | 135.55 | 63.86* | 119.27 | 102.96 | 35.34* |
| Pdzk1ip1 | 133.50 | 68.47 | 107.33 | 125.34 | 216.87 |
| Ptpro | 1.42 | 5.91 | 1.20 | 0.58 | 2.78 |
| Rpn1 | 108.33 | 90.37 | 90.52 | 80.59 | 87.08 |
| Scarb1 | 392.79 | 237.10 | 289.97 | 434.64 | 106.77* |
| Scd2 | 205.74 | 244.30 | 199.91 | 229.47 | 84.20 |
| Sec61a1 | 114.52 | 101.25 | 106.98 | 102.02 | 40.39 |
| Selp | 682.35 | 210.07* | 566.21 | 746.53 | 53.85* |
| Serinc3 | 523.01 | 476.27 | 552.93 | 434.22 | 177.98* |
| Serpinb10-ps | 176.75 | 79.74* | 150.23 | 142.55 | 46.67* |
| Serpinb2 | 1153.16 | 523.29* | 1393.18 | 1015.87 | 98.99* |
| Slc20a1 | 219.58 | 222.87 | 227.78 | 214.32 | 28.19* |
| Slc25a3 | 413.05 | 291.79 | 320.12 | 306.51 | 428.44 |
| Slc35d3 | 84.72 | 40.63 | 53.39 | 101.96 | 18.50* |
| Slc37a2 | 2.20 | 6.28 | 1.83 | 0.80 | 1.74 |
| Slc6a4 | 1717.84 | 542.43* | 1667.10 | 1172.70 | 71.01* |
| Ssr3 | 199.21 | 176.59 | 183.33 | 196.29 | 77.88 |
| Tmbim4 | 840.92 | 338.18* | 654.55 | 613.84 | 468.37 |
| Tmem40 | 363.85 | 145.47* | 267.51 | 294.62 | 264.04 |
| Tmem66 | 133.46 | 97.40 | 117.65 | 115.44 | 108.09 |
| Treml1 | 322.50 | 202.32 | 250.64 | 436.36 | 279.76 |
| Gene . | FPKM . | ||||
|---|---|---|---|---|---|
| WT . | SRF cKO . | MKL1 KO . | MKL2 cKO . | DKO . | |
| 1110008F13Rik | 168.39 | 108.66 | 124.53 | 118.02 | 162.05 |
| Alox5ap | 2324.05 | 1609.63 | 2435.91 | 2216.10 | 2811.82 |
| Atp5g3 | 385.29 | 244.15 | 250.03 | 242.72 | 508.98 |
| Bsg | 319.57 | 233.30 | 278.88 | 310.46 | 513.28 |
| Cd151 | 87.72 | 52.09 | 79.72 | 89.09 | 33.04* |
| Cd47 | 30.43 | 31.40 | 25.75 | 23.65 | 0.00 |
| Cd63 | 555.94 | 402.13 | 499.11 | 485.78 | 921.27 |
| Cd9 | 1630.72 | 852.78 | 1449.95 | 1627.59 | 619.83 |
| Cox4i1 | 521.67 | 401.89 | 414.34 | 408.12 | 1190.58 |
| Cox8a | 238.02 | 173.46 | 161.16 | 176.70 | 428.98 |
| Cyba | 155.52 | 323.36 | 134.54 | 87.42 | 765.77* |
| Dhcr24 | 148.52 | 76.21 | 124.12 | 133.68 | 32.72* |
| Dnajb4 | 42.37 | 25.26 | 36.92 | 47.77 | 11.62* |
| Esam | 126.13 | 68.79 | 80.62 | 146.31 | 91.92 |
| Eya2 | 31.29 | 14.01* | 41.15 | 24.88 | 10.51* |
| Fads2 | 207.86 | 130.48 | 172.31 | 254.37 | 50.26* |
| Fcer1g | 542.87 | 519.26 | 403.81 | 409.80 | 682.78 |
| Glipr1 | 245.40 | 205.21 | 238.57 | 161.53 | 227.67 |
| Gp1ba | 225.28 | 101.01* | 198.97 | 304.22 | 20.66* |
| Gp1bb | 68.88 | 31.31 | 47.20 | 51.64 | 73.57 |
| Gp49a | 812.14 | 447.61 | 810.08 | 724.34 | 192.64* |
| Gp5 | 270.36 | 99.97* | 183.32 | 242.73 | 103.53 |
| Gp9 | 307.39 | 118.95* | 222.33 | 264.54 | 212.19 |
| Gpr56 | 855.30 | 417.16 | 772.67 | 979.05 | 367.96 |
| H2-K1 | 602.78 | 843.12 | 527.17 | 490.93 | 1433.99* |
| Ifitm1 | 318.43 | 319.23 | 317.53 | 247.40 | 1536.24* |
| Ifitm2 | 263.50 | 278.33 | 236.67 | 250.93 | 501.76 |
| Ifitm3 | 580.84 | 578.90 | 410.03 | 463.05 | 1026.08 |
| Itga2b | 2506.71 | 1231.66* | 2305.33 | 2301.65 | 1637.24 |
| Itga6 | 665.79 | 277.35* | 625.44 | 736.21 | 50.74* |
| Itm2b | 691.15 | 965.94 | 734.92 | 600.35 | 739.78 |
| Laptm4a | 138.76 | 107.84 | 125.00 | 133.70 | 102.47 |
| Laptm5 | 2043.75 | 1786.38 | 2362.76 | 2407.72 | 512.81 |
| Lilrb4 | 1168.14 | 626.80 | 1169.76 | 1092.51 | 273.49* |
| Mcl1 | 112.29 | 129.28 | 127.35 | 88.38 | 57.66 |
| Mpl | 92.44 | 40.82* | 57.96 | 116.22 | 23.74* |
| Nrgn | 437.18 | 203.67 | 256.78 | 303.27 | 226.77 |
| P2rx1 | 135.55 | 63.86* | 119.27 | 102.96 | 35.34* |
| Pdzk1ip1 | 133.50 | 68.47 | 107.33 | 125.34 | 216.87 |
| Ptpro | 1.42 | 5.91 | 1.20 | 0.58 | 2.78 |
| Rpn1 | 108.33 | 90.37 | 90.52 | 80.59 | 87.08 |
| Scarb1 | 392.79 | 237.10 | 289.97 | 434.64 | 106.77* |
| Scd2 | 205.74 | 244.30 | 199.91 | 229.47 | 84.20 |
| Sec61a1 | 114.52 | 101.25 | 106.98 | 102.02 | 40.39 |
| Selp | 682.35 | 210.07* | 566.21 | 746.53 | 53.85* |
| Serinc3 | 523.01 | 476.27 | 552.93 | 434.22 | 177.98* |
| Serpinb10-ps | 176.75 | 79.74* | 150.23 | 142.55 | 46.67* |
| Serpinb2 | 1153.16 | 523.29* | 1393.18 | 1015.87 | 98.99* |
| Slc20a1 | 219.58 | 222.87 | 227.78 | 214.32 | 28.19* |
| Slc25a3 | 413.05 | 291.79 | 320.12 | 306.51 | 428.44 |
| Slc35d3 | 84.72 | 40.63 | 53.39 | 101.96 | 18.50* |
| Slc37a2 | 2.20 | 6.28 | 1.83 | 0.80 | 1.74 |
| Slc6a4 | 1717.84 | 542.43* | 1667.10 | 1172.70 | 71.01* |
| Ssr3 | 199.21 | 176.59 | 183.33 | 196.29 | 77.88 |
| Tmbim4 | 840.92 | 338.18* | 654.55 | 613.84 | 468.37 |
| Tmem40 | 363.85 | 145.47* | 267.51 | 294.62 | 264.04 |
| Tmem66 | 133.46 | 97.40 | 117.65 | 115.44 | 108.09 |
| Treml1 | 322.50 | 202.32 | 250.64 | 436.36 | 279.76 |
Data obtained from our RNA sequencing was compared to a list of the transmembrane proteins expressed specifically in megakaryocytes or up-regulated in megakaryocyte differentiation.24 Data are displayed in FPKM.
FPKM indicates fragments per kilobase of transcript per million; WT, wild type; SRF, serum response factor; KO, knockout; cKO, conditional knockout; and DKO, double knockout.
Differentially expressed genes reaching statistical significance (q < 0.05) compared to WT.