Top genes increased in both DKO and Srf Pf4-cKO megakaryocytes
| Gene . | FPKM . | ||||
|---|---|---|---|---|---|
| WT . | SRF cKO . | MKL1 KO . | MKL2 cKO . | DKO . | |
| Camp | 3.55 | 217.84 | 2.54 | 0.68 | 365.42 |
| Cd177 | 0.62 | 25.74 | 0.49 | 0.29 | 19.06 |
| Cebpe | 0.99 | 38.18 | 1.03 | 0.48 | 78.54 |
| Cldn15 | 2.83 | 50.78 | 3.05 | 1.93 | 51.11 |
| Cma1 | 0.26 | 15.85 | 0.25 | 0.14 | 24.63 |
| Cpa3 | 2.95 | 46.38 | 4.18 | 2.93 | 53.55 |
| Ctsg | 80.04 | 1110.46 | 77.54 | 42.09 | 1436.34 |
| Dmkn | 4.20 | 30.77 | 4.87 | 2.36 | 73.51 |
| Elane | 126.51 | 2657.87 | 129.82 | 76.19 | 4523.18 |
| Epx | 1.39 | 16.91 | 0.79 | 1.00 | 21.83 |
| Fcnb | 2.37 | 196.80 | 4.08 | 1.98 | 107.95 |
| G0s2 | 0.41 | 10.25 | 0.43 | 0.20 | 15.04 |
| Gfi1 | 1.78 | 70.69 | 2.11 | 1.07 | 39.86 |
| Gstm1 | 10.90 | 202.47 | 12.81 | 5.97 | 231.21 |
| Lcn2 | 46.34 | 447.48 | 55.93 | 24.45 | 774.36 |
| Ltf | 1.64 | 65.56 | 1.09 | 0.65 | 35.35 |
| Mapk13 | 0.66 | 12.56 | 0.54 | 0.29 | 18.51 |
| Mcpt8 | 7.46 | 141.38 | 10.37 | 6.24 | 362.91 |
| Mgst2 | 5.72 | 104.82 | 3.61 | 2.69 | 158.53 |
| Mogat2 | 0.48 | 33.16 | 0.44 | 0.46 | 19.44 |
| Ms4a3 | 33.51 | 348.80 | 38.93 | 19.18 | 483.45 |
| Ngp | 9.93 | 764.69 | 5.76 | 2.56 | 834.41 |
| Oscp1 | 0.74 | 10.77 | 0.75 | 0.74 | 13.66 |
| Pglyrp1 | 0.86 | 79.38 | 1.25 | 0.59 | 204.23 |
| Prg2 | 19.58 | 220.00 | 19.20 | 14.11 | 858.80 |
| Prg3 | 2.13 | 17.73 | 1.91 | 1.44 | 71.50 |
| Prss34 | 3.89 | 81.31 | 5.31 | 2.95 | 273.43 |
| Prss57 | 0.95 | 36.18 | 1.00 | 0.60 | 76.53 |
| Prtn3 | 123.47 | 1195.12 | 105.27 | 57.77 | 2720.61 |
| Ramp1 | 1.50 | 15.73 | 1.72 | 0.75 | 22.26 |
| S100a8 | 237.63 | 8015.96 | 226.82 | 68.84 | 9008.47 |
| S100a9 | 137.93 | 7049.68 | 144.50 | 44.80 | 9372.56 |
| Saa3 | 606.97 | 3825.61 | 628.93 | 244.01 | 9136.90 |
| Slpi | 30.75 | 244.71 | 36.52 | 17.42 | 663.24 |
| Tst | 1.56 | 15.88 | 1.14 | 1.26 | 35.82 |
| Gene . | FPKM . | ||||
|---|---|---|---|---|---|
| WT . | SRF cKO . | MKL1 KO . | MKL2 cKO . | DKO . | |
| Camp | 3.55 | 217.84 | 2.54 | 0.68 | 365.42 |
| Cd177 | 0.62 | 25.74 | 0.49 | 0.29 | 19.06 |
| Cebpe | 0.99 | 38.18 | 1.03 | 0.48 | 78.54 |
| Cldn15 | 2.83 | 50.78 | 3.05 | 1.93 | 51.11 |
| Cma1 | 0.26 | 15.85 | 0.25 | 0.14 | 24.63 |
| Cpa3 | 2.95 | 46.38 | 4.18 | 2.93 | 53.55 |
| Ctsg | 80.04 | 1110.46 | 77.54 | 42.09 | 1436.34 |
| Dmkn | 4.20 | 30.77 | 4.87 | 2.36 | 73.51 |
| Elane | 126.51 | 2657.87 | 129.82 | 76.19 | 4523.18 |
| Epx | 1.39 | 16.91 | 0.79 | 1.00 | 21.83 |
| Fcnb | 2.37 | 196.80 | 4.08 | 1.98 | 107.95 |
| G0s2 | 0.41 | 10.25 | 0.43 | 0.20 | 15.04 |
| Gfi1 | 1.78 | 70.69 | 2.11 | 1.07 | 39.86 |
| Gstm1 | 10.90 | 202.47 | 12.81 | 5.97 | 231.21 |
| Lcn2 | 46.34 | 447.48 | 55.93 | 24.45 | 774.36 |
| Ltf | 1.64 | 65.56 | 1.09 | 0.65 | 35.35 |
| Mapk13 | 0.66 | 12.56 | 0.54 | 0.29 | 18.51 |
| Mcpt8 | 7.46 | 141.38 | 10.37 | 6.24 | 362.91 |
| Mgst2 | 5.72 | 104.82 | 3.61 | 2.69 | 158.53 |
| Mogat2 | 0.48 | 33.16 | 0.44 | 0.46 | 19.44 |
| Ms4a3 | 33.51 | 348.80 | 38.93 | 19.18 | 483.45 |
| Ngp | 9.93 | 764.69 | 5.76 | 2.56 | 834.41 |
| Oscp1 | 0.74 | 10.77 | 0.75 | 0.74 | 13.66 |
| Pglyrp1 | 0.86 | 79.38 | 1.25 | 0.59 | 204.23 |
| Prg2 | 19.58 | 220.00 | 19.20 | 14.11 | 858.80 |
| Prg3 | 2.13 | 17.73 | 1.91 | 1.44 | 71.50 |
| Prss34 | 3.89 | 81.31 | 5.31 | 2.95 | 273.43 |
| Prss57 | 0.95 | 36.18 | 1.00 | 0.60 | 76.53 |
| Prtn3 | 123.47 | 1195.12 | 105.27 | 57.77 | 2720.61 |
| Ramp1 | 1.50 | 15.73 | 1.72 | 0.75 | 22.26 |
| S100a8 | 237.63 | 8015.96 | 226.82 | 68.84 | 9008.47 |
| S100a9 | 137.93 | 7049.68 | 144.50 | 44.80 | 9372.56 |
| Saa3 | 606.97 | 3825.61 | 628.93 | 244.01 | 9136.90 |
| Slpi | 30.75 | 244.71 | 36.52 | 17.42 | 663.24 |
| Tst | 1.56 | 15.88 | 1.14 | 1.26 | 35.82 |
A list of genes more than 16-fold increased in DKO and Srf Pf4-cKO megakaryocytes compared to WT megakaryocytes. Data are displayed in FPKM.
FPKM indicates fragments per kilobase of transcript per million; WT, wild type; SRF, serum response factor; KO, knockout; cKO, conditional knockout; and DKO, double knockout.