Effect of combination treatment with Peg-IFNα 2a and Nutlin-3 on the JAK2 genotype of PV hematopoietic colonies
| Patient no. . | Granulocyte JAK2 V617F allele burden, % . | Additions to culture . | |||||
|---|---|---|---|---|---|---|---|
| None . | Peg IFNα 2a 200 U/mL + Nutlin-3 200nM . | ||||||
| Homo, % . | Hetero, % . | WT, % . | Homo, % . | Hetero, % . | WT, % . | ||
| PV 1 | 53 | 29.2 | 33.3 | 37.5 | 42 | 8 | 50 |
| PV 2 | 27 | 0 | 25 | 75 | 0 | 9 | 91 |
| PV 3 | 43.2 | 4.1 | 16.7 | 79.2 | 2.1 | 4.2 | 93.7 |
| PV 4 | 78 | 54.2 | 25 | 20.8 | 30 | 20 | 50 |
| PV 5 | 20 | 38.6 | 29.6 | 31.8 | 40.5 | 14.3 | 45.2 |
| PV 6 | 60 | 0 | 43 | 57 | 0 | 32.4 | 67.6 |
| Patient no. . | Granulocyte JAK2 V617F allele burden, % . | Additions to culture . | |||||
|---|---|---|---|---|---|---|---|
| None . | Peg IFNα 2a 200 U/mL + Nutlin-3 200nM . | ||||||
| Homo, % . | Hetero, % . | WT, % . | Homo, % . | Hetero, % . | WT, % . | ||
| PV 1 | 53 | 29.2 | 33.3 | 37.5 | 42 | 8 | 50 |
| PV 2 | 27 | 0 | 25 | 75 | 0 | 9 | 91 |
| PV 3 | 43.2 | 4.1 | 16.7 | 79.2 | 2.1 | 4.2 | 93.7 |
| PV 4 | 78 | 54.2 | 25 | 20.8 | 30 | 20 | 50 |
| PV 5 | 20 | 38.6 | 29.6 | 31.8 | 40.5 | 14.3 | 45.2 |
| PV 6 | 60 | 0 | 43 | 57 | 0 | 32.4 | 67.6 |
At least 50% of colonies were examined if the number of colonies exceeded 50 in an individual dish. If the number of colonies in an individual dish were < 40, 90% of colonies were plucked and examined.
Homo indicates JAK2V617F homozygous; Hetero, JAK2V617F heterozygous; and WT, contains only wild-type JAK2.