Prime candidate genes for the determination and stabilization of the B-lymphoid-myeloid biphenotypic state of cellular development
| Accession no. . | UniGene no. . | Description . | FC of 0 ng/mL Dox vs Pax5−/− rtTA . | FC of 0 ng/mL Dox vs 10 ng/mL Dox . | FC of 0 ng/mL Dox vs 300 ng/mL Dox . | FC of 0 ng/mL Dox vs 1000 ng/mL Dox . |
|---|---|---|---|---|---|---|
| NM_009844 | Mm.4360 | CD19 antigen | 1.20 | −1.14 | 3.98 | 8.68 |
| NM_007655 | Mm.1355 | CD79A antigen (immunoglobulin-associated alpha) | −2.26 | −1.85 | 1.15 | 3.59 |
| NM_008528 | Mm.9746 | BLNK, B-cell linker | −11.56 | −1.77 | 6.07 | 18.78 |
| NM_008714 | Mm.290610 | Notch1, Notch gene homolog 1 (Drosophila) | −2.90 | 1.01 | −2.51 | −6.96 |
| NM_010496 | Mm.34871 | Id2, inhibitor of DNA-binding 2 | −1.05 | 1.03 | −2.85 | −3.85 |
| NM_009970 | Mm.287228 | Csf2ra, Mus musculus colony-stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) | 2.78 | 2.28 | 2.20 | −1.16 |
| NM_008091 | Mm.313866 | Gata3, GATA-binding protein 3 | 2.83 | 2.02 | 1.37 | −1.01 |
| BC058161 | Mm.349667 | Cebpa, CCAAT/enhancer-binding protein (C/EBP), alpha | 1.40 | 1.70 | 1.50 | −1.44 |
| NM_007679 | Mm.347407 | Cebpd, CCAAT/enhancer-binding protein (C/EBP), delta | 1.65 | 1.87 | 1.59 | −1.39 |
| NM_008037 | Mm.24684 | Fosl2, Fos-like antigen 2 | 1.63 | 2.30 | 2.80 | −1.15 |
| NM_011355 | Mm.1302 | PU.1, Sfpi1, SFFV proviral integration 1 | −2.00 | −1.19 | −2.49 | −1.65 |
| NM_008091 | Mm.313866 | GATA3, GATA-binding protein 3 | −2.83 | −2.02 | −1.37 | −1.01 |
| NM_07779 | Mm.22574 | Csf1r, Mcsf1r (macrophage), colony-stimulating factor 1 receptor | −1.45 | −1.85 | −3.10 | −1.67 |
| Accession no. . | UniGene no. . | Description . | FC of 0 ng/mL Dox vs Pax5−/− rtTA . | FC of 0 ng/mL Dox vs 10 ng/mL Dox . | FC of 0 ng/mL Dox vs 300 ng/mL Dox . | FC of 0 ng/mL Dox vs 1000 ng/mL Dox . |
|---|---|---|---|---|---|---|
| NM_009844 | Mm.4360 | CD19 antigen | 1.20 | −1.14 | 3.98 | 8.68 |
| NM_007655 | Mm.1355 | CD79A antigen (immunoglobulin-associated alpha) | −2.26 | −1.85 | 1.15 | 3.59 |
| NM_008528 | Mm.9746 | BLNK, B-cell linker | −11.56 | −1.77 | 6.07 | 18.78 |
| NM_008714 | Mm.290610 | Notch1, Notch gene homolog 1 (Drosophila) | −2.90 | 1.01 | −2.51 | −6.96 |
| NM_010496 | Mm.34871 | Id2, inhibitor of DNA-binding 2 | −1.05 | 1.03 | −2.85 | −3.85 |
| NM_009970 | Mm.287228 | Csf2ra, Mus musculus colony-stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) | 2.78 | 2.28 | 2.20 | −1.16 |
| NM_008091 | Mm.313866 | Gata3, GATA-binding protein 3 | 2.83 | 2.02 | 1.37 | −1.01 |
| BC058161 | Mm.349667 | Cebpa, CCAAT/enhancer-binding protein (C/EBP), alpha | 1.40 | 1.70 | 1.50 | −1.44 |
| NM_007679 | Mm.347407 | Cebpd, CCAAT/enhancer-binding protein (C/EBP), delta | 1.65 | 1.87 | 1.59 | −1.39 |
| NM_008037 | Mm.24684 | Fosl2, Fos-like antigen 2 | 1.63 | 2.30 | 2.80 | −1.15 |
| NM_011355 | Mm.1302 | PU.1, Sfpi1, SFFV proviral integration 1 | −2.00 | −1.19 | −2.49 | −1.65 |
| NM_008091 | Mm.313866 | GATA3, GATA-binding protein 3 | −2.83 | −2.02 | −1.37 | −1.01 |
| NM_07779 | Mm.22574 | Csf1r, Mcsf1r (macrophage), colony-stimulating factor 1 receptor | −1.45 | −1.85 | −3.10 | −1.67 |
Microarray analysis was performed on total RNA extracted from Pax5−/− huPax5 pro-/pre-B-cell clone 4, induced to express huPax5 at different levels of doxycycline (0, 10, 300, and 1000 ng/mL) in IL-7/OP9 for 3 days in vitro. Presented are characteristic examples for the differential expression of B-, T-, NK-, and myeloid-lineage–related genes at different levels of doxycycline.
FC indicates fold change; Dox, doxycycline; and rtTA, retroviral transactivator.