Characteristics of untreated RBCs and RBCs treated with increasing concentrations of LPC (1-15μM)
| Sample . | Hb, g/dL . | MCHC, g/dL . | MCH, pg . | MCV, μm3 . | Projected area, μm2 . | A/V, μm−1 . | Sphericity . | EImax, a.u. . | Spleen retention, % . |
|---|---|---|---|---|---|---|---|---|---|
| Untreated RBCs | 11.3 (1.8) | 34 (2) | 31 (3) | 92 (2) | 71 (1) | 0.77 (0.01) | 0.81 (0.01) | 0.638 (0.01) | 9 (4) |
| LPC-treated RBCs | |||||||||
| 1-2.5μM | 10.5 (0.8) | 35 (2) | 32 (3) | 92 (1) | 70 (0.3) | 0.76 (0.01) | 0.81 (0.02) | 0.621 (0.01) | 19 (7) |
| 3.5-5.0μM | 10.1 (0.3) | 35 (1) | 33 (1) | 94 (0) | 63 (3) | 0.67 (0.03) | 0.91 (0.03) | 0.520 (0.04) | 64 (16) |
| 6.0-7.5μM | 9.7 (0.2) | 33 (2) | 32 (1) | 97 (1) | 59 (3) | 0.61 (0.04) | 0.92 (0.02) | 0.470 (0.05) | 79 (13) |
| 8.5-15.0μM | 10.1 (0.1) | 32 (1) | 30 (2) | 95 (0) | 56 (3) | 0.59 (0.03) | 0.93 (0.01) | 0.423 (0.04) | 92 (4) |
| Sample . | Hb, g/dL . | MCHC, g/dL . | MCH, pg . | MCV, μm3 . | Projected area, μm2 . | A/V, μm−1 . | Sphericity . | EImax, a.u. . | Spleen retention, % . |
|---|---|---|---|---|---|---|---|---|---|
| Untreated RBCs | 11.3 (1.8) | 34 (2) | 31 (3) | 92 (2) | 71 (1) | 0.77 (0.01) | 0.81 (0.01) | 0.638 (0.01) | 9 (4) |
| LPC-treated RBCs | |||||||||
| 1-2.5μM | 10.5 (0.8) | 35 (2) | 32 (3) | 92 (1) | 70 (0.3) | 0.76 (0.01) | 0.81 (0.02) | 0.621 (0.01) | 19 (7) |
| 3.5-5.0μM | 10.1 (0.3) | 35 (1) | 33 (1) | 94 (0) | 63 (3) | 0.67 (0.03) | 0.91 (0.03) | 0.520 (0.04) | 64 (16) |
| 6.0-7.5μM | 9.7 (0.2) | 33 (2) | 32 (1) | 97 (1) | 59 (3) | 0.61 (0.04) | 0.92 (0.02) | 0.470 (0.05) | 79 (13) |
| 8.5-15.0μM | 10.1 (0.1) | 32 (1) | 30 (2) | 95 (0) | 56 (3) | 0.59 (0.03) | 0.93 (0.01) | 0.423 (0.04) | 92 (4) |
All data are means (SD). Hb, MCHC, MCH, and MCV were measured with ADVIA 120 Hematology System.
Hb indicates hemoglobin; MCHC, mean corpuscular hemoglobin concentration; MCH, mean corpuscular hemoglobin; MCV, mean cell volume; EImax, EI at a shear stress of 30 Pascals; and a.u., arbitary units.