Hematologic parameters of WT and BERK mice as well as WTBM and BERKBM mice treated with 1H1 rat anti–mouse TF or control rat IgG antibodies
| Variable . | WT . | BERK . | WTBM/IgG . | WTBM/1H1 . | BERKBM/IgG . | BERKBM/1H1 . |
|---|---|---|---|---|---|---|
| RBCs, 106/μL | 8.3 ± 0.9 | 4.7 ± 0.6 | 7.2 ± 0.6* | 7.0 ± 0.5 | 4.8 ± 0.3††† | 5.2 ± 0.5‡‡‡ |
| Hemoglobin, g/dL | 9.1 ± 0.8 | 4.2 ± 0.8 | 7.1 ± 0.4*** | 7.2 ± 0.3 | 4.6 ± 0.3††† | 4.7 ± 0.4‡‡‡ |
| Hematocrit, % | 36.9 ± 1.1 | 21.4 ± 2.6 | 30.2 ± 2.1*** | 30.7 ± 1.2 | 23.1 ± 1.6††† | 24.1 ± 2.1‡‡‡ |
| MCV, fL | 45.4 ± 4.9 | 45.7 ± 4.3 | 42.2 ± 4.5 | 44.2 ± 3.4 | 47.9 ± 2.4††† | 46.1 ± 2.4 |
| MCH, pg | NA | NA | 9.9 ± 0.3 | 10.5 ± 0.6 | 9.6 ± 0.9 | 9.3 ± 0.8‡ |
| MCHC, g/dL | NA | NA | 24.1 ± 2.4 | 24.0 ± 1.9 | 20.2 ± 1.5††† | 20.1 ± 1.4‡‡ |
| RDW, % | 18.3 ± 3.9 | 31.5 ± 2.9 | 18.9 ± 3.2 | 18.0 ± 2.7 | 32.2 ± 2.2††† | 33.2 ± 1.9‡‡‡ |
| WBCs, 103/μL | 4.4 ± 2.4 | 17.3 ± 5.4 | 8.4 ± 2.9** | 8.1 ± 6.3 | 17.0 ± 8.0†† | 16.9 ± 6.0‡ |
| Neutrophils, 103/μL | 0.8 ± 0.5 | 3.7 ± 1.5 | 1.2 ± 0.3 | 1.1 ± 0.5 | 3.3 ± 1.4††† | 3.2 ± 1.2‡‡ |
| Lymphocytes, 103/μL | 3.3 ± 1.8 | 12.5 ± 5.5 | 6.6 ± 2.4** | 6.3 ± 5.8 | 12.4 ± 6.5† | 12.6 ± 5.0 |
| Monocytes, 103/μL | 0.2 ± 0.2 | 0.9 ± 0.3 | 0.5 ± 0.2* | 0.5 ± 0.1 | 1.2 ± 0.5††† | 1.1 ± 0.4‡ |
| Platelets, 103/μL | 682 ± 100 | 727 ± 125 | 712 ± 109 | 716 ± 62 | 770 ± 132 | 795 ± 118 |
| Variable . | WT . | BERK . | WTBM/IgG . | WTBM/1H1 . | BERKBM/IgG . | BERKBM/1H1 . |
|---|---|---|---|---|---|---|
| RBCs, 106/μL | 8.3 ± 0.9 | 4.7 ± 0.6 | 7.2 ± 0.6* | 7.0 ± 0.5 | 4.8 ± 0.3††† | 5.2 ± 0.5‡‡‡ |
| Hemoglobin, g/dL | 9.1 ± 0.8 | 4.2 ± 0.8 | 7.1 ± 0.4*** | 7.2 ± 0.3 | 4.6 ± 0.3††† | 4.7 ± 0.4‡‡‡ |
| Hematocrit, % | 36.9 ± 1.1 | 21.4 ± 2.6 | 30.2 ± 2.1*** | 30.7 ± 1.2 | 23.1 ± 1.6††† | 24.1 ± 2.1‡‡‡ |
| MCV, fL | 45.4 ± 4.9 | 45.7 ± 4.3 | 42.2 ± 4.5 | 44.2 ± 3.4 | 47.9 ± 2.4††† | 46.1 ± 2.4 |
| MCH, pg | NA | NA | 9.9 ± 0.3 | 10.5 ± 0.6 | 9.6 ± 0.9 | 9.3 ± 0.8‡ |
| MCHC, g/dL | NA | NA | 24.1 ± 2.4 | 24.0 ± 1.9 | 20.2 ± 1.5††† | 20.1 ± 1.4‡‡ |
| RDW, % | 18.3 ± 3.9 | 31.5 ± 2.9 | 18.9 ± 3.2 | 18.0 ± 2.7 | 32.2 ± 2.2††† | 33.2 ± 1.9‡‡‡ |
| WBCs, 103/μL | 4.4 ± 2.4 | 17.3 ± 5.4 | 8.4 ± 2.9** | 8.1 ± 6.3 | 17.0 ± 8.0†† | 16.9 ± 6.0‡ |
| Neutrophils, 103/μL | 0.8 ± 0.5 | 3.7 ± 1.5 | 1.2 ± 0.3 | 1.1 ± 0.5 | 3.3 ± 1.4††† | 3.2 ± 1.2‡‡ |
| Lymphocytes, 103/μL | 3.3 ± 1.8 | 12.5 ± 5.5 | 6.6 ± 2.4** | 6.3 ± 5.8 | 12.4 ± 6.5† | 12.6 ± 5.0 |
| Monocytes, 103/μL | 0.2 ± 0.2 | 0.9 ± 0.3 | 0.5 ± 0.2* | 0.5 ± 0.1 | 1.2 ± 0.5††† | 1.1 ± 0.4‡ |
| Platelets, 103/μL | 682 ± 100 | 727 ± 125 | 712 ± 109 | 716 ± 62 | 770 ± 132 | 795 ± 118 |
No statistically significant differences were observed in hematologic profile between the following groups: BERK vs BERKBM/IgG, WTBM/IgG vs WTBM/1H1, and BERKBM/IgG vs BERKBM/1H1.
RBCs indicates red blood cells; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; NA, not applicable; MCHC, mean corpuscular hemoglobin concentration; and WBCs, white blood cells.
Statistical difference in hematologic parameters between WT and WTBM/IgG mice: *P < .05; **P < .01; and ***P < .001.
Statistical difference in hematologic parameters between WTBM and BERKBM mice treated with control rat IgG antibody: †P < .05; ††P < .01; and †††P < .001.
Statistical difference in hematologic parameters between WTBM and BERKBM mice treated with rat anti–mouse TF antibody: ‡P < .05; ‡‡P < .01; and ‡‡‡P < .001.