Table 2 Direct phospholipid binding... | American Society of Hematology

Table 2

Direct phospholipid binding measurements

FVIII mutants		FV mutants
Mutant	K_{D, μM}	Mutant	K_{D, μM}
FVIII_WT	0.46 ± 0.08	FV_WT	0.7 ± 0.2
FVIII_WW	0.19 ± 0.03	FV_MF	—
FVIII_LS	0.33 ± 0.10	FV_LL	1.4 ± 0.1
FVIII_WW/LS	0.19 ± 0.05	FV_MF/LL	0.65 ± 0.06

FVIII mutants		FV mutants
Mutant	K_{D, μM}	Mutant	K_{D, μM}
FVIII_WT	0.46 ± 0.08	FV_WT	0.7 ± 0.2
FVIII_WW	0.19 ± 0.03	FV_MF	—
FVIII_LS	0.33 ± 0.10	FV_LL	1.4 ± 0.1
FVIII_WW/LS	0.19 ± 0.05	FV_MF/LL	0.65 ± 0.06

— indicates that the signal was too small to fit, probably because of low FV_MF concentration (see supplemental Figure 1B).