LSK cells and CDPs were isolated from BM or day-3 FL-stimulated BM from UBC-GFP mice.¹⁴  Single LSK cells were sorted and cultured with total wild-type BM feeder cells and FL for 8 days, and single CDPs were sorted and cultured with wild-type CDP feeder cells and FL for 5 days. At the end of culture, the single progenitors that had given rise to clonal progeny were identified by observation under UV fluorescence. These clones were analyzed by flow cytometry for the presence of CD11c⁺CD45RA⁻ cDCs and CD11c⁺CD45RA⁺ pDCs. Fifteen percent of LSK cells formed DC clones and 93 were analyzed; 51% of CDP formed DC clones and 99 were analyzed. Clones were classified as pDC-containing clones if the progeny included any pDCs or as pDC-restricted clones if the progeny were solely pDCs. Results are shown as the percentage of total cells.