Effects of different concentrations of erythropoietin on the in vitro growth of JAK2V617F+ ECs
| Incubation time, h . | Erythropoietin concentration, IU/mL . | |||
|---|---|---|---|---|
| 0 . | 0.1 . | 1 . | 10 . | |
| 0 | 1 × 103 | 1 × 103 | 1 × 103 | 1 × 103 |
| 12 | 0.98 × 103 | 0.99 × 103 | 1.01 × 103 | 0.99 × 103 |
| 24 | 1.04 × 103 | 1.02 × 103 | 1.1 × 103 | 1.06 × 103 |
| 48 | 1.34 × 103 | 1.28 × 103 | 1.31 × 103 | 1.53 × 103 |
| 72 | 1.57 × 103 | 1.62 × 103 | 1.88 × 103 | 2.01 × 103 |
| 96 | 1.91 × 103 | 1.87 × 103 | 2.06 × 103 | 2.49 × 103 |
| Incubation time, h . | Erythropoietin concentration, IU/mL . | |||
|---|---|---|---|---|
| 0 . | 0.1 . | 1 . | 10 . | |
| 0 | 1 × 103 | 1 × 103 | 1 × 103 | 1 × 103 |
| 12 | 0.98 × 103 | 0.99 × 103 | 1.01 × 103 | 0.99 × 103 |
| 24 | 1.04 × 103 | 1.02 × 103 | 1.1 × 103 | 1.06 × 103 |
| 48 | 1.34 × 103 | 1.28 × 103 | 1.31 × 103 | 1.53 × 103 |
| 72 | 1.57 × 103 | 1.62 × 103 | 1.88 × 103 | 2.01 × 103 |
| 96 | 1.91 × 103 | 1.87 × 103 | 2.06 × 103 | 2.49 × 103 |
Results are the mean of the total cell number recovered from the cultures of the mutated ECs of the 2 patients tested (patients 6 and 17) with increasing doses of erythropoietin at different time points (see “Methods” for details).