B-cell populations in KSHV latency locus transgenic mice
| . | . | Frequency . | Absolute numbers . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wild-type . | KSHV latency tg . | . | Wild-type . | KSHV latency tg . | |||||||||
| Class . | Marker . | % . | SD . | n . | % . | SD . | n . | P . | # . | SD . | # . | SD . | P . |
| Mature | CD19+IgM+IgD+ | 22.08 | 7.34 | 9 | 36.00 | 6.36 | 9 | 0.01 | 3717 | 1182 | 6197 | 1710 | 0.003 |
| Activated B | CD19+IgM+IgD+FSC-hi | 4.82 | 2.14 | 9 | 9.54 | 2.44 | 9 | 0.0003 | 783 | 326 | 1859 | 555 | 0.0002 |
| MZ | CD19+IgM+IgD- | 11.83 | 3.12 | 9 | 15.75 | 2.21 | 9 | 0.01 | 1974 | 390 | 3028 | 376 | 0.00003 |
| Activated MZ | CD19+IgM+IgD-FSC-hi | 2.57 | 1.14 | 9 | 4.47 | 1.48 | 9 | 0.009 | 429 | 160 | 857 | 276 | 0.001 |
| MZ | CD19+CD21hiCD23-CD24+ | 2.25 | 0.23 | 6 | 3.69 | 1.01 | 9 | 0.002 | 352 | 36 | 592 | 168 | 0.002 |
| MZ | CD19+CD21hiCD23-IgD- | 1.07 | 0.32 | 6 | 1.75 | 0.63 | 9 | 0.02 | 182 | 53 | 281 | 111 | 0.04 |
| GC | CD19+IgD-CD21-CD23+ | 0.46 | 0.27 | 6 | 0.35 | 0.13 | 9 | NS | 72 | 42 | 56 | 20 | NS |
| NF/T1 | CD19+CD21intCD23-IgDlo | 1.52 | 0.45 | 6 | 1.31 | 0.38 | 9 | NS | 241 | 71 | 210 | 59 | NS |
| T2-FP | CD19+IgD+CD21intCD23+ | 31.24 | 5.75 | 6 | 28.84 | 6.62 | 9 | NS | 4980 | 944 | 4627 | 1048 | NS |
| T2-MZP | CD19+IgD+CD23+CD21hi | 4.12 | 0.90 | 6 | 5.41 | 1.09 | 9 | 0.03 | 655 | 135 | 867 | 170 | 0.02 |
| FO | CD19+IgD+IgMloCD23+ | 4.24 | 0.97 | 7 | 4.05 | 1.50 | 11 | NS | 672 | 147 | 696 | 235 | NS |
| B-1a | CD19+B220+CD5+ | 0.52 | 0.29 | 9 | 0.76 | 0.27 | 9 | NS | 111 | 50 | 116 | 53 | NS |
| Plasma (spleen) | CD19-B220-CD138+ | 0.89 | 0.19 | 5 | 1.32 | 0.28 | 5 | 0.02 | 504 | 86 | 1106 | 152 | 0.0002 |
| Plasma (BM) | CD19-B220-CD138+ | 0.17 | 0.07 | 6 | 0.47 | 0.18 | 6 | 0.004 | 37 | 12 | 115 | 44 | 0.006 |
| . | . | Frequency . | Absolute numbers . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wild-type . | KSHV latency tg . | . | Wild-type . | KSHV latency tg . | |||||||||
| Class . | Marker . | % . | SD . | n . | % . | SD . | n . | P . | # . | SD . | # . | SD . | P . |
| Mature | CD19+IgM+IgD+ | 22.08 | 7.34 | 9 | 36.00 | 6.36 | 9 | 0.01 | 3717 | 1182 | 6197 | 1710 | 0.003 |
| Activated B | CD19+IgM+IgD+FSC-hi | 4.82 | 2.14 | 9 | 9.54 | 2.44 | 9 | 0.0003 | 783 | 326 | 1859 | 555 | 0.0002 |
| MZ | CD19+IgM+IgD- | 11.83 | 3.12 | 9 | 15.75 | 2.21 | 9 | 0.01 | 1974 | 390 | 3028 | 376 | 0.00003 |
| Activated MZ | CD19+IgM+IgD-FSC-hi | 2.57 | 1.14 | 9 | 4.47 | 1.48 | 9 | 0.009 | 429 | 160 | 857 | 276 | 0.001 |
| MZ | CD19+CD21hiCD23-CD24+ | 2.25 | 0.23 | 6 | 3.69 | 1.01 | 9 | 0.002 | 352 | 36 | 592 | 168 | 0.002 |
| MZ | CD19+CD21hiCD23-IgD- | 1.07 | 0.32 | 6 | 1.75 | 0.63 | 9 | 0.02 | 182 | 53 | 281 | 111 | 0.04 |
| GC | CD19+IgD-CD21-CD23+ | 0.46 | 0.27 | 6 | 0.35 | 0.13 | 9 | NS | 72 | 42 | 56 | 20 | NS |
| NF/T1 | CD19+CD21intCD23-IgDlo | 1.52 | 0.45 | 6 | 1.31 | 0.38 | 9 | NS | 241 | 71 | 210 | 59 | NS |
| T2-FP | CD19+IgD+CD21intCD23+ | 31.24 | 5.75 | 6 | 28.84 | 6.62 | 9 | NS | 4980 | 944 | 4627 | 1048 | NS |
| T2-MZP | CD19+IgD+CD23+CD21hi | 4.12 | 0.90 | 6 | 5.41 | 1.09 | 9 | 0.03 | 655 | 135 | 867 | 170 | 0.02 |
| FO | CD19+IgD+IgMloCD23+ | 4.24 | 0.97 | 7 | 4.05 | 1.50 | 11 | NS | 672 | 147 | 696 | 235 | NS |
| B-1a | CD19+B220+CD5+ | 0.52 | 0.29 | 9 | 0.76 | 0.27 | 9 | NS | 111 | 50 | 116 | 53 | NS |
| Plasma (spleen) | CD19-B220-CD138+ | 0.89 | 0.19 | 5 | 1.32 | 0.28 | 5 | 0.02 | 504 | 86 | 1106 | 152 | 0.0002 |
| Plasma (BM) | CD19-B220-CD138+ | 0.17 | 0.07 | 6 | 0.47 | 0.18 | 6 | 0.004 | 37 | 12 | 115 | 44 | 0.006 |
Absolute number of the gated cells.
hi, high; int, intermediate; lo, low; NF/T1, newly formed or transitional type 1; NS, not significant; T2-FP, transitional type 2-follicular precursor B cell; SD, standard deviation; tg, transgenic.