Frequency and TGF-β1 immunostaining of MKs in the spleen of normal donors and PMF patients and wild-type and Gata1low mice
| . | BM . | Spleen . | ||
|---|---|---|---|---|
| Frequency MK/mm2 . | TGF-β1 content/MK count difference, % . | Frequency MK/mm2 . | TGF-β1 content/MK count difference, % . | |
| Normal donor | 98.0 ± 34.62 | 0.5 ± 0.1 | 2.7 ± 2.7 | 11.7 ± 2.7 |
| PMF patients | 435.8 ± 28.1* | 17.1 ± 2.4* | 151.2 ± 70.6* | 34.9 ± 2.1* |
| Wild-type mice | 320.0 ± 35.8 | 6.4 ± 1.2 | 93.3 ± 20.1 | 3.2 ± 0.3 |
| Gata1low mice untreated | 920.6 ± 136.8* | 20.2 ± 2.0* | 146.7 ± 20.1* | 24.4 ± 2.2* |
| Gata1low vehicle treated | 880.1 ± 110.3* | 18.9 ± 0.8 | 584.0 ± 60.1* | 24.3 ± 0.9* |
| Gata1lowSB431542 treated | 504.0 ± 29.9*† | 5.3 ± 1.1† | 296.0 ± 54.1*† | 9.2 ± 0.7† |
| . | BM . | Spleen . | ||
|---|---|---|---|---|
| Frequency MK/mm2 . | TGF-β1 content/MK count difference, % . | Frequency MK/mm2 . | TGF-β1 content/MK count difference, % . | |
| Normal donor | 98.0 ± 34.62 | 0.5 ± 0.1 | 2.7 ± 2.7 | 11.7 ± 2.7 |
| PMF patients | 435.8 ± 28.1* | 17.1 ± 2.4* | 151.2 ± 70.6* | 34.9 ± 2.1* |
| Wild-type mice | 320.0 ± 35.8 | 6.4 ± 1.2 | 93.3 ± 20.1 | 3.2 ± 0.3 |
| Gata1low mice untreated | 920.6 ± 136.8* | 20.2 ± 2.0* | 146.7 ± 20.1* | 24.4 ± 2.2* |
| Gata1low vehicle treated | 880.1 ± 110.3* | 18.9 ± 0.8 | 584.0 ± 60.1* | 24.3 ± 0.9* |
| Gata1lowSB431542 treated | 504.0 ± 29.9*† | 5.3 ± 1.1† | 296.0 ± 54.1*† | 9.2 ± 0.7† |
MK frequency was determined on 5 randomly chosen sections and TGF-β immunostaining by Methamorph analyses on 5-7 randomly chosen MKs per smear. MK frequencies and TGF-β immunostaining are presented as mean (±SD) of values observed in the BM from 6 PMF patients and 4 normal donors, the spleen from 10 PMF patients and 4 normal donors, and BM and spleen of 3-5 Gata1low animals per experimental group. Results statistically different between nondiseased and diseased samples (P < .01) or between SB431542- and vehicle-treated Gata1low mice (P < .05) are indicated by * and †, respectively.