Table 6 Frequency of progenitor cells,... | American Society of Hematology

Table 6

Frequency of progenitor cells, erythroid cells, and MK precursors in the BM and spleen of vehicle- and SB431542-treated Gata1^low mice

	Wild type	Gata1^low
	Wild type	Untreated	Vehicle treated	SB431542 treated
CD117^pos
BM	9.8 ± 3.5	3.5 ± 1.2*	6.5 ± 1.9	7.8 ± 1.1†
Spleen	0.2 ± 0.5	4.6 ± 2.1*	6.6 ± 2.1	11.5 ± 2.1†
CD71^pos/Ter119^pos, %
BM	19.3 ± 8.1	13.9 ± 4.3	14.7 ± 1.6	26.7 ± 2.8†
Spleen	8.5 ± 2.9	14.2 ± 5.5*	12.7 ± 3.6	13.2 ± 2.3
CD41^pos/CD61^neg, %
BM	9.9 ± 5.8	30.5 ± 8.7*	30.9 ± 3.1	28.8 ± 4.6
Spleen	12.8 ± 5.7	23.7 ± 3.1*	b.d.‡	b.d.‡
CD41^pos/CD61^pos, %
BM	3.8 ± 1.0	8.1 ± 2.5*	14.5 ± 4.7	10.6 ± 3.4
Spleen	2.3 ± 1.1	19.4 ± 2.3*	16.7 ± 3.4	10.7 ± 3.0‡

	Wild type	Gata1^low
	Wild type	Untreated	Vehicle treated	SB431542 treated
CD117^pos
BM	9.8 ± 3.5	3.5 ± 1.2*	6.5 ± 1.9	7.8 ± 1.1†
Spleen	0.2 ± 0.5	4.6 ± 2.1*	6.6 ± 2.1	11.5 ± 2.1†
CD71^pos/Ter119^pos, %
BM	19.3 ± 8.1	13.9 ± 4.3	14.7 ± 1.6	26.7 ± 2.8†
Spleen	8.5 ± 2.9	14.2 ± 5.5*	12.7 ± 3.6	13.2 ± 2.3
CD41^pos/CD61^neg, %
BM	9.9 ± 5.8	30.5 ± 8.7*	30.9 ± 3.1	28.8 ± 4.6
Spleen	12.8 ± 5.7	23.7 ± 3.1*	b.d.‡	b.d.‡
CD41^pos/CD61^pos, %
BM	3.8 ± 1.0	8.1 ± 2.5*	14.5 ± 4.7	10.6 ± 3.4
Spleen	2.3 ± 1.1	19.4 ± 2.3*	16.7 ± 3.4	10.7 ± 3.0‡

Data are presented as mean (±SD) of 3-6 determinations per experimental point. MK precursors were defined as CD41^pos cells and divided into immature (CD61^neg) and mature (CD61^pos) MKs. *, †, and ‡, P < 0.05 between untreated Gata1^low and wild-type mice, SB431542- and vehicle-treated Gata1^low mice, or treated and untreated Gata1^low mice, respectively. The cloning efficiency of CD117^pos cells prospectively isolated from vehicle- and SB431542-treated animals was 55 ± 12 vs 44 ± 5 CFC/10³ plated cells, respectively.

b.d., below detection.

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