Definitions of BCR-ABL1 mutations
| BCR-ABL1 mutation classification . | Definition . |
|---|---|
| Predominant mutation | Mutation detectable by direct Sanger sequencing of PCR amplicon pool |
| Single mutation | Single missense mutation detectable in a sample subjected to Sanger sequencing of PCR amplicon pool |
| Double mutation | Two missense mutations detectable in a sample subjected to Sanger sequencing of PCR amplicon pool |
| Low-level mutations | Mutation detected only by sequencing of an individually cloned PCR amplicon |
| (1) Add-on mutation | Missense mutation emerging in addition to predominant mutation |
| (2) Independent low-level mutation | Missense mutations present in separate clones relative to clones harboring the predominant mutation(s) |
| Compound mutation | Two or more missense mutations in the same sample and the same BCR-ABL1 molecule (Figure 1) |
| Polyclonal mutation | Two or more missense mutations in the same sample, but different BCR-ABL1 molecules (Figure 1) |
| Component | A mutation that is part of a compound mutation |
| BCR-ABL1 mutation classification . | Definition . |
|---|---|
| Predominant mutation | Mutation detectable by direct Sanger sequencing of PCR amplicon pool |
| Single mutation | Single missense mutation detectable in a sample subjected to Sanger sequencing of PCR amplicon pool |
| Double mutation | Two missense mutations detectable in a sample subjected to Sanger sequencing of PCR amplicon pool |
| Low-level mutations | Mutation detected only by sequencing of an individually cloned PCR amplicon |
| (1) Add-on mutation | Missense mutation emerging in addition to predominant mutation |
| (2) Independent low-level mutation | Missense mutations present in separate clones relative to clones harboring the predominant mutation(s) |
| Compound mutation | Two or more missense mutations in the same sample and the same BCR-ABL1 molecule (Figure 1) |
| Polyclonal mutation | Two or more missense mutations in the same sample, but different BCR-ABL1 molecules (Figure 1) |
| Component | A mutation that is part of a compound mutation |