Table 3 Studies investigating... | American Society of Hematology

Table 3

Studies investigating long-distance intercellular miRNA communication

Authors	Analyzed aspect	Cells/cell lines	transported miRNAs	Methods	Reference
Yuan et al, 2009	Transfer of miRNAs by embryonic stem cell microvesicles	Murine ES cells	miR-290	Coculture, qRT-PCR, visual confirmation of GFP-labeled MV uptake	60
		MEFs	miR-291-3p
			miR-292-3p
			miR-294, -295
Zhang et al, 2010	Influence of secreted monocytic miR-150 on endothelial cell migration	THP-1 primary PBMCs	miR-150 and 26 others	Endothelial cell migration and various reporter assays, quantification of miRNA target via western blot	24
Zhang et al, 2010		HMEC-1	miR-150 and 26 others		24
Collino et al, 2010	miRNA content of MSC and HLSC vesicles	Primary MSCs and HLSCs	miR-21, -451,	qRT-PCR of producer cells, MVs, and target cells; visual confirmation of MV and miRNA uptake, quantification of miRNA targets	70
		mTEC	miR-100, -99a
			miR-223, -564
Vickers et al, 2011	Cellular export and uptake of functional HDL-bound miRNAs	Huh7, HEK293, J774, BHK cells, human plasma	miR-135a*	miRNA microarray and luciferase reporter assays, loss/gain of function studies	19
			miR-188-5p
			miR-877, -223
Grange et al, 2011	Exchange of genetic information between tumor and stromal cells	HUVECs	miR-29a/c, -19b,	MV treatment of SCID mice, in vitro + in vivo angiogenesis + metastasis assays, mRNA/miRNA profiling	71
		CD105⁺ cancer stem cells	miR-151, -650,
			miR-200c, -141
			miR-92
Hergenreider et al, 2012	Atheroprotective communication between endothelial cells and SMCs through miRNAs	HUVECs	miR-143	Coculture and reporter assays, qRT-PCR, injection of MVs into ApoE^-/^- mice + readout, antagomiR experiments	72
		HAECs	miR-145
		HASMCs
Umezu et al, 2012	Leukemia cell to endothelial cell communication via exosomal miRNAs	K562	miR-92a	Coculture and reporter assays, miRNA expression profiling	67
Umezu et al, 2012		HUVECs	miR-92a	Coculture and reporter assays, miRNA expression profiling	67
Fonsato et al, 2012	Antitumor effect of miRNA-containing vesicles on hepatoma cells	HLSCs	miR-451	Intratumor injection of miRNA and miRNA inhibitor–containing MVs into SCID mice	73
		HepG2	miR-223
		Primary hepatoma	miR-24
			miR-31
Xin et al, 2012	Communication between MSCs and neural cells via exosomal miRNAs	Primary rat MSCs, neurons, and astrocytes	miR-133b	Coculture, knockdown, and luciferase reporter assays, in vitro treatment of neurons with MVs	74
Ismail et al, 2013	Transport of macrophage-derived MVs to target cells	Primary PBMCs	miR-223	AntagomiR transfection, fluorescent MV labeling + microscopy, luciferase reporter assay, qRT-PCR	75
		THP-1
		CCL-204
		A549
Cantaluppi et al, 2012	miRNA-containing MVs released by endothelial cells preventing kidney injury	Primary PBMCs	miR-126	Injection of MVs into rats following IRI (also Dicer knocked-down/antagomiR MV application)	76
Cantaluppi et al, 2012		TepCs	miR-296		76
Montecalvo et al, 2012	Transfer of functional miRNAs between murine DCs via exosomes	Murine BM-derived DCs and 1H3.1 and OT-II T cells	miR-451, miR148a	Fluorescence- and microscopy-based analysis of MV uptake and cytosolic content release, miRNA profiling + reporter assays	77
Katsman et al, 2012	Effect of ES cell MVs on retinal progenitor cells	Murine ES cells	miR-292	qRT-PCR	78
Katsman et al, 2012	Effect of ES cell MVs on retinal progenitor cells	MIO-M1	miR-295	qRT-PCR	78

Authors	Analyzed aspect	Cells/cell lines	transported miRNAs	Methods	Reference
Yuan et al, 2009	Transfer of miRNAs by embryonic stem cell microvesicles	Murine ES cells	miR-290	Coculture, qRT-PCR, visual confirmation of GFP-labeled MV uptake	60
		MEFs	miR-291-3p
			miR-292-3p
			miR-294, -295
Zhang et al, 2010	Influence of secreted monocytic miR-150 on endothelial cell migration	THP-1 primary PBMCs	miR-150 and 26 others	Endothelial cell migration and various reporter assays, quantification of miRNA target via western blot	24
Zhang et al, 2010		HMEC-1	miR-150 and 26 others		24
Collino et al, 2010	miRNA content of MSC and HLSC vesicles	Primary MSCs and HLSCs	miR-21, -451,	qRT-PCR of producer cells, MVs, and target cells; visual confirmation of MV and miRNA uptake, quantification of miRNA targets	70
		mTEC	miR-100, -99a
			miR-223, -564
Vickers et al, 2011	Cellular export and uptake of functional HDL-bound miRNAs	Huh7, HEK293, J774, BHK cells, human plasma	miR-135a*	miRNA microarray and luciferase reporter assays, loss/gain of function studies	19
			miR-188-5p
			miR-877, -223
Grange et al, 2011	Exchange of genetic information between tumor and stromal cells	HUVECs	miR-29a/c, -19b,	MV treatment of SCID mice, in vitro + in vivo angiogenesis + metastasis assays, mRNA/miRNA profiling	71
		CD105⁺ cancer stem cells	miR-151, -650,
			miR-200c, -141
			miR-92
Hergenreider et al, 2012	Atheroprotective communication between endothelial cells and SMCs through miRNAs	HUVECs	miR-143	Coculture and reporter assays, qRT-PCR, injection of MVs into ApoE^-/^- mice + readout, antagomiR experiments	72
		HAECs	miR-145
		HASMCs
Umezu et al, 2012	Leukemia cell to endothelial cell communication via exosomal miRNAs	K562	miR-92a	Coculture and reporter assays, miRNA expression profiling	67
Umezu et al, 2012		HUVECs	miR-92a	Coculture and reporter assays, miRNA expression profiling	67
Fonsato et al, 2012	Antitumor effect of miRNA-containing vesicles on hepatoma cells	HLSCs	miR-451	Intratumor injection of miRNA and miRNA inhibitor–containing MVs into SCID mice	73
		HepG2	miR-223
		Primary hepatoma	miR-24
			miR-31
Xin et al, 2012	Communication between MSCs and neural cells via exosomal miRNAs	Primary rat MSCs, neurons, and astrocytes	miR-133b	Coculture, knockdown, and luciferase reporter assays, in vitro treatment of neurons with MVs	74
Ismail et al, 2013	Transport of macrophage-derived MVs to target cells	Primary PBMCs	miR-223	AntagomiR transfection, fluorescent MV labeling + microscopy, luciferase reporter assay, qRT-PCR	75
		THP-1
		CCL-204
		A549
Cantaluppi et al, 2012	miRNA-containing MVs released by endothelial cells preventing kidney injury	Primary PBMCs	miR-126	Injection of MVs into rats following IRI (also Dicer knocked-down/antagomiR MV application)	76
Cantaluppi et al, 2012		TepCs	miR-296		76
Montecalvo et al, 2012	Transfer of functional miRNAs between murine DCs via exosomes	Murine BM-derived DCs and 1H3.1 and OT-II T cells	miR-451, miR148a	Fluorescence- and microscopy-based analysis of MV uptake and cytosolic content release, miRNA profiling + reporter assays	77
Katsman et al, 2012	Effect of ES cell MVs on retinal progenitor cells	Murine ES cells	miR-292	qRT-PCR	78
Katsman et al, 2012	Effect of ES cell MVs on retinal progenitor cells	MIO-M1	miR-295	qRT-PCR	78

BM, bone marrow; DC, dendritic cell; IRI, renal ischemia-reperfusion injury; MSC, mesenchymal stem cell; MV, microvesicle; PBMC, peripheral blood mononuclear cell; SCID, severe combined immunodeficiency; GFP, green fluorescent protein.

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