Table 2 Tetanus toxin–specific antibody... | American Society of Hematology

Table 2

Tetanus toxin–specific antibody production by different B-cell subsets 7 d postvaccination with Tedivax Pro Adulto

	CD3⁻CD19⁺ CD20⁺CD27⁺CD43⁻			CD3⁻CD19⁺CD20⁺ CD27⁺CD43⁺CD69⁻			CD3⁻CD19⁺ CD20⁻CD27⁺CD43⁺
	IgA	IgG	IgM	IgA	IgG	IgM	IgA	IgG	IgM
Donor 1	0	0	0	0	40	0	0	82	1
Donor 2	ND	ND	ND	5	47	0	15	84	0
Donor 3	0	1	0	0	13	0	5	45	0
Donor 4	0	0	0	1	61	0	3	53	1

	CD3⁻CD19⁺ CD20⁺CD27⁺CD43⁻			CD3⁻CD19⁺CD20⁺ CD27⁺CD43⁺CD69⁻			CD3⁻CD19⁺ CD20⁻CD27⁺CD43⁺
	IgA	IgG	IgM	IgA	IgG	IgM	IgA	IgG	IgM
Donor 1	0	0	0	0	40	0	0	82	1
Donor 2	ND	ND	ND	5	47	0	15	84	0
Donor 3	0	1	0	0	13	0	5	45	0
Donor 4	0	0	0	1	61	0	3	53	1

Memory B cells (CD3⁻CD19⁺CD20⁺CD27⁺CD43⁻), CD3⁻CD19⁺CD20⁺CD27⁺CD43⁺CD69⁻ B cells, and plasmablasts (CD3⁻CD19⁺CD20⁻CD43⁺CD27⁺) from 3 healthy donors were purified by a combination of magnetic cell isolation and cell sorting (according to the gating strategy shown in Figure 1). The cells were plated at 2.5 × 10³ cells per well, incubated at 37°C for 12 h, and analyzed for tetanus toxin–specific antibody secretion by ELISPOT. ND, not done.

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