Table 2 List of primers (5′-3′) used... | American Society of Hematology

Table 2

List of primers (5′-3′) used

Mutagenesis

M420delF: GCAGCCTGCCTCGTCATTTTTATCTCACCTGACC

M420delR: GGTCAGGTGAGATAAAAATGACGAGGCAGGCTGC

M408F: GCATCTACCCCATGGCCATGTCAAATTTGTTGGCG

M408R: CGCCAACAAATTTGACATGGCCATGGGGTAGATGC

PCR pyrosequencing

SLC22A1-F: GGCCATGTCAAATTTGTT

SLC22A1-R (biotin labelled): GTTGTCTGCTTTCATCATTTC

SLC22A1-S (Pyrosequencing): CGGGGGCAGCCTGCCTC

PCR direct sequencing

OCT634F(PCR & Sequencing): CATCACCATTGACCGCGTGG

OCT762R: CCCACACTTCGATTGCCTGG

mRNA quantification and sequencing

OCT3F: GGGCAGCCTGCCTCGTCATG

OCT3FN: GGGCAGCCTGCCTCGTCAT

OCT1503R: ACCTCCCTCAGCCTGAAGAC

OCT1181F (sequencing): TCATCACCATTGACCGCGTG

OCT1413R (sequencing): 5TCCCACCTATGTCACACAGG

F indicates forward primers; and R, reverse primers. Sequencing primers are also indicated.