Cloning and mutagenesis primers
| Application . | Gene . | . | 5′-3′ sequence . |
|---|---|---|---|
| Cloning | Distal mouse PIT1 promoter | Forward | CAACGCGTGCGGCCATCATATAGAGAGCA |
| Reverse | AGAATTCCAAGGGCAAGTCTCAGCACAC | ||
| Cloning | Proximal PIT1 promoter | Forward | AGAATTCGGGACATGGGAACACTCACTG |
| Reverse | CATAGATCTCAGCAACTCCAAGGCAAGGT | ||
| Mutagenesis | EKLF1 | Forward | GTGTTTGGATATGCTAGTTTAGGGGAGCCTTACACG |
| Reverse | CGTGTAAGGCTCCCCTAAACTAGCATATCCAAACAC | ||
| Mutagenesis | EKLF2 | Forward | CTGGGAACGCAAGCCTATCC |
| Reverse | TACCTCGCCCATCTCACGTG | ||
| Mutagenesis | EKLF2 | Forward | CACGTGAGATGGGCGAGGTA |
| Reverse | GGGCGCGCCTAGCCG |
| Application . | Gene . | . | 5′-3′ sequence . |
|---|---|---|---|
| Cloning | Distal mouse PIT1 promoter | Forward | CAACGCGTGCGGCCATCATATAGAGAGCA |
| Reverse | AGAATTCCAAGGGCAAGTCTCAGCACAC | ||
| Cloning | Proximal PIT1 promoter | Forward | AGAATTCGGGACATGGGAACACTCACTG |
| Reverse | CATAGATCTCAGCAACTCCAAGGCAAGGT | ||
| Mutagenesis | EKLF1 | Forward | GTGTTTGGATATGCTAGTTTAGGGGAGCCTTACACG |
| Reverse | CGTGTAAGGCTCCCCTAAACTAGCATATCCAAACAC | ||
| Mutagenesis | EKLF2 | Forward | CTGGGAACGCAAGCCTATCC |
| Reverse | TACCTCGCCCATCTCACGTG | ||
| Mutagenesis | EKLF2 | Forward | CACGTGAGATGGGCGAGGTA |
| Reverse | GGGCGCGCCTAGCCG |