Subjects with multiple Ig Isotypes and/or IgG subclasses of FVIII-binding antibodies
| No. (%) of positive samples that contain multiple Ig isotypes/IgG subclasses, identification code . | Titer of FVIII-binding antibodies . | |||||
|---|---|---|---|---|---|---|
| IgG1 . | IgG2 . | IgG3 . | IgG4 . | IgA . | IgM . | |
| Healthy: 7 of 116 positives (6%), sample size: n = 600 | ||||||
| Healthy 12 | neg | neg | 1:80 | neg | neg | 1:20 |
| Healthy 54 | neg | neg | 1:20 | neg | neg | 1:40 |
| Healthy 93 | 1:20 | neg | neg | neg | 1:20 | neg |
| Healthy 111 | 1:20 | neg | neg | neg | 1:40 | neg |
| Healthy 297 | 1:40 | 1:40 | ||||
| Healthy 311 | 1:40 | 1:40 | ||||
| Healthy 595 | 1:20 | 1:40 | ||||
| Hemophilia A without inhibitor: 5 of 26 positives (19%), sample size: n = 77 | ||||||
| HA-noINH 13 | 1:80 | 1:40 | ||||
| HA-noINH 28 | 1:20 | 1:20 | ||||
| HA-noINH 55 | 1:40 | 1:40 | ||||
| HA-noINH 63 | 1:40 | 1:20 | ||||
| HA-noINH 77 | 1:80 | 1:20 | ||||
| Hemophilia A with inhibitor: 19 of 20 positives (95%), sample size: n = 20 | ||||||
| HA-INH 1 | 1:80 | 1:320 | 1:20 | |||
| HA-INH 2 | 1:80 | 1:640 | ||||
| HA-INH 3 | 1:80 | 1:640 | ||||
| HA-INH 4 | 1:320 | 1:160 | 1:80 | 1:1280 | ||
| HA-INH 5 | 1:40 | 1:1280 | ||||
| HA-INH 6 | 1:160 | 1:1280 | ||||
| HA-INH 7 | 1:640 | 1:20 | 1:5120 | |||
| HA-INH 8 | 1:2560 | 1:320 | 1:40 | 1:10 240 | ||
| HA-INH 9 | 1:1280 | 1:5120 | 1:40 | |||
| HA-INH 10 | 1:160 | 1:40 | 1:80 | 1:10 240 | ||
| HA-INH 11 | 1:5120 | 1:640 | 1:163840 | 1:20 | ||
| HA-INH 12 | 1:80 | 1:20 | 1:1280 | |||
| HA-INH 14 | 1:80 | 1:40 | ||||
| HA-INH 15 | 1:20 | 1:160 | ||||
| HA-INH 16 | 1:80 | 1:40 | 1:320 | |||
| HA-INH 17 | 1:80 | 1:20 | 1:160 | 1:320 | ||
| HA-INH 18 | 1:80 | 1:320 | ||||
| HA-INH 19 | 1:160 | 1:320 | ||||
| HA-INH 20 | 1:80 | 1:40 | 1:640 | |||
| Acquired hemophilia A: 9 of 9 positives (100%), sample size: n = 9 | ||||||
| Acqu-HA1 | 1:5120 | 1:40 | 1:20 480 | 1:20 | ||
| Acqu-HA 2 | 1:1280 | 1:320 | ||||
| Acqu-HA 3 | 1:2560 | 1:80 | 1:640 | |||
| Acqu-HA 4 | 1:320 | 1:1280 | ||||
| Acqu-HA 5 | 1:10 240 | 1:320 | 1:80 | 1:2560 | ||
| Acqu-HA 6 | 1:40 960 | 1:160 | 1:320 | 1:2560 | ||
| Acqu-HA 7 | 1:5120 | 1:20 | 1:5120 | 1:40 | ||
| Acqu-HA 8 | 1:2560 | 1:5120 | ||||
| Acqu-HA 9 | 1:1280 | 1:80 | 1:20 480 | |||
| Hemophilia A after successful ITI: 1 of 9 positives (11%), sample size: n = 23 | ||||||
| HA-ITI 11 | 1:40 | 1:40 | ||||
| No. (%) of positive samples that contain multiple Ig isotypes/IgG subclasses, identification code . | Titer of FVIII-binding antibodies . | |||||
|---|---|---|---|---|---|---|
| IgG1 . | IgG2 . | IgG3 . | IgG4 . | IgA . | IgM . | |
| Healthy: 7 of 116 positives (6%), sample size: n = 600 | ||||||
| Healthy 12 | neg | neg | 1:80 | neg | neg | 1:20 |
| Healthy 54 | neg | neg | 1:20 | neg | neg | 1:40 |
| Healthy 93 | 1:20 | neg | neg | neg | 1:20 | neg |
| Healthy 111 | 1:20 | neg | neg | neg | 1:40 | neg |
| Healthy 297 | 1:40 | 1:40 | ||||
| Healthy 311 | 1:40 | 1:40 | ||||
| Healthy 595 | 1:20 | 1:40 | ||||
| Hemophilia A without inhibitor: 5 of 26 positives (19%), sample size: n = 77 | ||||||
| HA-noINH 13 | 1:80 | 1:40 | ||||
| HA-noINH 28 | 1:20 | 1:20 | ||||
| HA-noINH 55 | 1:40 | 1:40 | ||||
| HA-noINH 63 | 1:40 | 1:20 | ||||
| HA-noINH 77 | 1:80 | 1:20 | ||||
| Hemophilia A with inhibitor: 19 of 20 positives (95%), sample size: n = 20 | ||||||
| HA-INH 1 | 1:80 | 1:320 | 1:20 | |||
| HA-INH 2 | 1:80 | 1:640 | ||||
| HA-INH 3 | 1:80 | 1:640 | ||||
| HA-INH 4 | 1:320 | 1:160 | 1:80 | 1:1280 | ||
| HA-INH 5 | 1:40 | 1:1280 | ||||
| HA-INH 6 | 1:160 | 1:1280 | ||||
| HA-INH 7 | 1:640 | 1:20 | 1:5120 | |||
| HA-INH 8 | 1:2560 | 1:320 | 1:40 | 1:10 240 | ||
| HA-INH 9 | 1:1280 | 1:5120 | 1:40 | |||
| HA-INH 10 | 1:160 | 1:40 | 1:80 | 1:10 240 | ||
| HA-INH 11 | 1:5120 | 1:640 | 1:163840 | 1:20 | ||
| HA-INH 12 | 1:80 | 1:20 | 1:1280 | |||
| HA-INH 14 | 1:80 | 1:40 | ||||
| HA-INH 15 | 1:20 | 1:160 | ||||
| HA-INH 16 | 1:80 | 1:40 | 1:320 | |||
| HA-INH 17 | 1:80 | 1:20 | 1:160 | 1:320 | ||
| HA-INH 18 | 1:80 | 1:320 | ||||
| HA-INH 19 | 1:160 | 1:320 | ||||
| HA-INH 20 | 1:80 | 1:40 | 1:640 | |||
| Acquired hemophilia A: 9 of 9 positives (100%), sample size: n = 9 | ||||||
| Acqu-HA1 | 1:5120 | 1:40 | 1:20 480 | 1:20 | ||
| Acqu-HA 2 | 1:1280 | 1:320 | ||||
| Acqu-HA 3 | 1:2560 | 1:80 | 1:640 | |||
| Acqu-HA 4 | 1:320 | 1:1280 | ||||
| Acqu-HA 5 | 1:10 240 | 1:320 | 1:80 | 1:2560 | ||
| Acqu-HA 6 | 1:40 960 | 1:160 | 1:320 | 1:2560 | ||
| Acqu-HA 7 | 1:5120 | 1:20 | 1:5120 | 1:40 | ||
| Acqu-HA 8 | 1:2560 | 1:5120 | ||||
| Acqu-HA 9 | 1:1280 | 1:80 | 1:20 480 | |||
| Hemophilia A after successful ITI: 1 of 9 positives (11%), sample size: n = 23 | ||||||
| HA-ITI 11 | 1:40 | 1:40 | ||||
FVIII indicates factor VIII; ITI, immune tolerance induction therapy; and neg, titer < 1:20.