Observations associated with PD-1 expression on T cells in B-cell lymphomas
| Reference . | No. of Patients . | Antibody (manufacturer) . | Associations . |
|---|---|---|---|
| HL | |||
| 41 | 19 | (BD Pharmingen) | PD-1 levels in the peripheral blood T cells of HL patients tended to be higher in active disease and declined along with treatment; PD-1 and FOXP3 seemed to be alternatively expressed (expressed in different patients). |
| 42 | 189 | AF 1086 (R&D Systems) | PD-1+ cell density was lower in patients with 9p24.1 gains (near PDL2 locus) and in those with high numbers of FOXP3+ Tregs but positively correlated with densities of granzyme B+ and TIA-1+ cells. |
| 61 | 280 | 760-4448 (Ventana Roche) | There was no correlation between 9p24.1 alterations, EBV status, and rosetting of PD-1+ T cells in HL. |
| FL | |||
| 47 | 132 | NAT105 (Abcam) | PD-1 expression in FL tissues correlated with low grade, good performance status, and low LDH; PD-1 expression was alternative to FOXP3 positivity in T cells (expressed in different patients). |
| 46 | 49 | Polyclonal AF1086 (R&D Systems) | The amount of PD-1+ TILs was higher in FL grade 1 to 2 than in FL grade 3 and further decreased in FL with transformation to DLBCL. |
| 51 | 91 | NAT (Abcam) | The number of PD-1+ cells in FL tissues correlated with the number of FOXP3+ Tregs. |
| 55 | 82 | NAT, abcam52587 (Abcam) | PD-1 positivity in FL biopsies was associated with male sex and high serum β2M levels. |
| 48 | 14 | clone EH12.1 | High PD-1 expression levels in TILs correlated with decreased cytokine signaling response, including IL-4–induced phosphorylation of STAT6 and IL-10/IL-21–induced phosphorylation of STAT3. |
| 52 | 32 | ab52587 (Abcam) | Intratumoral PD-1high T cells: CD4+CXCR5+BCL-6+TIM-3−; PD-1low T cells: TIM-3+BCL-6−CXCR5−. |
| CLL | |||
| 69 | 77 | (BD Biosciences) | CLL patients with inverted CD4:CD8 ratios had significantly increased PD-1 levels on CD8+ T cells in CLL blood samples. |
| DLBCL | |||
| 56 | 70 | Mouse mAb | Number of PD-1high (TFH) cells positively correlated with number of CD4+ T cells. |
| 57 | 236 | Clone NAT105 (Abcam) | The number of PD-1+ TILs was lower in groups of patients with B symptoms, extranodal sites, bulky masses, ABC subtype, and PD-L1+ (≥30%) DLBCL. |
| 64 | 46 | (eBioscience) | Increased blood PD-1 levels were associated with stage III and IV, B symptoms, IPI >2, elevated LDH, and high Ki-67; PD-1 levels decreased after 4 courses of chemotherapy. |
| 61 | 253 | 760-4448 (Ventana Roche) | Tumor PD-L1 expression levels positively correlated with the amount of PD-1+ TILs in ABC-DLBCL. |
| 58 | 126 | Clone MRQ-22 (Cell Marque) | The number of PD-1+ TILs positively correlated with PD-L1 expression levels on tumor cells and/or macrophages. |
| 62 | 31 | An increased number of PD-1+ TILs negatively correlated with tumor Ki-67 and positively correlated with PD-L1 expression in histiocytes but not with PD-L1 on tumor cells. | |
| 65 | 60 | Clone MIH4 (phycoerythrin) | PD-1 expression in tumor biopsies did not correlate with PD-1 expression in the peripheral blood. |
| Reference . | No. of Patients . | Antibody (manufacturer) . | Associations . |
|---|---|---|---|
| HL | |||
| 41 | 19 | (BD Pharmingen) | PD-1 levels in the peripheral blood T cells of HL patients tended to be higher in active disease and declined along with treatment; PD-1 and FOXP3 seemed to be alternatively expressed (expressed in different patients). |
| 42 | 189 | AF 1086 (R&D Systems) | PD-1+ cell density was lower in patients with 9p24.1 gains (near PDL2 locus) and in those with high numbers of FOXP3+ Tregs but positively correlated with densities of granzyme B+ and TIA-1+ cells. |
| 61 | 280 | 760-4448 (Ventana Roche) | There was no correlation between 9p24.1 alterations, EBV status, and rosetting of PD-1+ T cells in HL. |
| FL | |||
| 47 | 132 | NAT105 (Abcam) | PD-1 expression in FL tissues correlated with low grade, good performance status, and low LDH; PD-1 expression was alternative to FOXP3 positivity in T cells (expressed in different patients). |
| 46 | 49 | Polyclonal AF1086 (R&D Systems) | The amount of PD-1+ TILs was higher in FL grade 1 to 2 than in FL grade 3 and further decreased in FL with transformation to DLBCL. |
| 51 | 91 | NAT (Abcam) | The number of PD-1+ cells in FL tissues correlated with the number of FOXP3+ Tregs. |
| 55 | 82 | NAT, abcam52587 (Abcam) | PD-1 positivity in FL biopsies was associated with male sex and high serum β2M levels. |
| 48 | 14 | clone EH12.1 | High PD-1 expression levels in TILs correlated with decreased cytokine signaling response, including IL-4–induced phosphorylation of STAT6 and IL-10/IL-21–induced phosphorylation of STAT3. |
| 52 | 32 | ab52587 (Abcam) | Intratumoral PD-1high T cells: CD4+CXCR5+BCL-6+TIM-3−; PD-1low T cells: TIM-3+BCL-6−CXCR5−. |
| CLL | |||
| 69 | 77 | (BD Biosciences) | CLL patients with inverted CD4:CD8 ratios had significantly increased PD-1 levels on CD8+ T cells in CLL blood samples. |
| DLBCL | |||
| 56 | 70 | Mouse mAb | Number of PD-1high (TFH) cells positively correlated with number of CD4+ T cells. |
| 57 | 236 | Clone NAT105 (Abcam) | The number of PD-1+ TILs was lower in groups of patients with B symptoms, extranodal sites, bulky masses, ABC subtype, and PD-L1+ (≥30%) DLBCL. |
| 64 | 46 | (eBioscience) | Increased blood PD-1 levels were associated with stage III and IV, B symptoms, IPI >2, elevated LDH, and high Ki-67; PD-1 levels decreased after 4 courses of chemotherapy. |
| 61 | 253 | 760-4448 (Ventana Roche) | Tumor PD-L1 expression levels positively correlated with the amount of PD-1+ TILs in ABC-DLBCL. |
| 58 | 126 | Clone MRQ-22 (Cell Marque) | The number of PD-1+ TILs positively correlated with PD-L1 expression levels on tumor cells and/or macrophages. |
| 62 | 31 | An increased number of PD-1+ TILs negatively correlated with tumor Ki-67 and positively correlated with PD-L1 expression in histiocytes but not with PD-L1 on tumor cells. | |
| 65 | 60 | Clone MIH4 (phycoerythrin) | PD-1 expression in tumor biopsies did not correlate with PD-1 expression in the peripheral blood. |
Anti-PD-1 clone names were not available in references 41, 64, and 69.
IPI, International Prognostic Index; LDH, lactate dehydrogenase.