Peptide mapping of plasminogen glycated by glucose
Tryptic peptide . | Sequence . | Mass (Da) . | Peptide detected . | Normalized intensity with respect to unglycated plasminogen . | |
---|---|---|---|---|---|
Rt (min) . | Molecular ion (m/z) . | ||||
T17 | NGITCQK | 819.4 | 42.2 | 410.7 (M+2)/2 | 0.60 ± 0.13 |
Glycated peptide | NGITCQKFLWSSTSPHRPR | 2173.0 | 58.7 | 1087.5 (M+2)/2 | |
T16-(FL)T17 | 725.3 (M+3)/3 | ||||
T64 | CPGR | 488.2 | 31.7 | 489.2 (M+1) | 0.70 ± 0.14 |
Glycated peptide | KFLCPGR | 778.3 | — | Not detected | |
T63-(FL)-T64 |
Tryptic peptide . | Sequence . | Mass (Da) . | Peptide detected . | Normalized intensity with respect to unglycated plasminogen . | |
---|---|---|---|---|---|
Rt (min) . | Molecular ion (m/z) . | ||||
T17 | NGITCQK | 819.4 | 42.2 | 410.7 (M+2)/2 | 0.60 ± 0.13 |
Glycated peptide | NGITCQKFLWSSTSPHRPR | 2173.0 | 58.7 | 1087.5 (M+2)/2 | |
T16-(FL)T17 | 725.3 (M+3)/3 | ||||
T64 | CPGR | 488.2 | 31.7 | 489.2 (M+1) | 0.70 ± 0.14 |
Glycated peptide | KFLCPGR | 778.3 | — | Not detected | |
T63-(FL)-T64 |
Cysteine residues have an S-acetamido modification. Peptide responses were normalized to peptide T83 as internal standard (FVTWIEGVJR), retention time 61.6 minutes, ion detected m/z = 627.3 [(M+2)/2]. Rt, retention time.