Immunophenotyping of disease progression
| . | % of Total cells . | |||
|---|---|---|---|---|
| GFP+ . | GFP+/CD11b+ . | GFP+/CD3+ . | GFP+/B220+ . | |
| Day 14 | ||||
| Vector (n = 5) | 11.49 ± 1.36 | 5.99 ± 1.35 | 0.46 ± 0.04 | 1.24 ± 0.37 |
| BCR/ABL (n = 5) | 30.31 ± 9.17 | 28.15 ± 8.41 | 0.20 ± 0.07 | 1.08 ± 0.61 |
| ΔUBD (n = 5) | 26.07 ± 3.34 | 23.84 ± 1.20 | 0.26 ± 0.02 | 1.29 ± 1.03 |
| Day 21 | ||||
| Vector (n = 5) | 7.46 ± 0.92 | 3.17 ± 0.72 | 0.21 ± 0.17 | 1.06 ± 1.17 |
| BCR/ABL (n = 5) | 63.09 ± 12.00 | 38.62 ± 12.00 | 0.92 ± 1.45 | 5.17 ± 1.62 |
| ΔUBD (n = 5) | 44.43 ± 15.28 | 19.35 ± 2.09 | 0.79 ± 0.51 | 4.62 ± 3.65 |
| Day 25 | ||||
| Vector (n = 5) | 9.59 ± 5.93 | 4.14 ± 1.45 | 0.14 ± 0.08 | 1.68 ± 1.29 |
| BCR/ABL (n = 5) | 64.50 ± 9.25 | 55.34 ± 8.34 | 0.25 ± 0.09 | 4.42 ± 3.12 |
| ΔUBD (n = 5) | 39.46 ± 19.52 | 18.64 ± 8.46 | 0.59 ± 0.21 | 2.25 ± 1.43 |
| Day 28 | ||||
| Vector (n = 5) | 11.22 ± 3.95 | 5.37 ± 3.09 | 0.66 ± 0.35 | 2.76 ± 0.75 |
| BCR/ABL (n = 5) | 87.10 ± 2.65 | 76.04 ± 5.10 | 0.37 ± 0.17 | 4.26 ± 2.05 |
| ΔUBD (n = 5) | 80.16 ± 5.90 | 42.25 ± 17.11 | 0.64 ± 0.29 | 5.28 ± 1.99 |
| . | % of Total cells . | |||
|---|---|---|---|---|
| GFP+ . | GFP+/CD11b+ . | GFP+/CD3+ . | GFP+/B220+ . | |
| Day 14 | ||||
| Vector (n = 5) | 11.49 ± 1.36 | 5.99 ± 1.35 | 0.46 ± 0.04 | 1.24 ± 0.37 |
| BCR/ABL (n = 5) | 30.31 ± 9.17 | 28.15 ± 8.41 | 0.20 ± 0.07 | 1.08 ± 0.61 |
| ΔUBD (n = 5) | 26.07 ± 3.34 | 23.84 ± 1.20 | 0.26 ± 0.02 | 1.29 ± 1.03 |
| Day 21 | ||||
| Vector (n = 5) | 7.46 ± 0.92 | 3.17 ± 0.72 | 0.21 ± 0.17 | 1.06 ± 1.17 |
| BCR/ABL (n = 5) | 63.09 ± 12.00 | 38.62 ± 12.00 | 0.92 ± 1.45 | 5.17 ± 1.62 |
| ΔUBD (n = 5) | 44.43 ± 15.28 | 19.35 ± 2.09 | 0.79 ± 0.51 | 4.62 ± 3.65 |
| Day 25 | ||||
| Vector (n = 5) | 9.59 ± 5.93 | 4.14 ± 1.45 | 0.14 ± 0.08 | 1.68 ± 1.29 |
| BCR/ABL (n = 5) | 64.50 ± 9.25 | 55.34 ± 8.34 | 0.25 ± 0.09 | 4.42 ± 3.12 |
| ΔUBD (n = 5) | 39.46 ± 19.52 | 18.64 ± 8.46 | 0.59 ± 0.21 | 2.25 ± 1.43 |
| Day 28 | ||||
| Vector (n = 5) | 11.22 ± 3.95 | 5.37 ± 3.09 | 0.66 ± 0.35 | 2.76 ± 0.75 |
| BCR/ABL (n = 5) | 87.10 ± 2.65 | 76.04 ± 5.10 | 0.37 ± 0.17 | 4.26 ± 2.05 |
| ΔUBD (n = 5) | 80.16 ± 5.90 | 42.25 ± 17.11 | 0.64 ± 0.29 | 5.28 ± 1.99 |
Immunophenotyping was performed as described in Methods.