CD8+ T-LGL leukemia patient characteristics for TCR repertoire deep sequencing (N = 11)
| Cohort ID . | STAT3 mutation . | Hematologic presentation . | AAD . | Sex . | Viral serology . | Autoimmune disease . | Therapy . | Flow VB ID . | Clone size* . | Absolute major clone size† . | HLA A . | HLA A2 . | HLA B . | HLA B2 . | HLA Cw . | HLA Cw 2 . | HLA DRB1 . | HLA DRB1 2 . | HLA DQB1 . | HLA DQB1 2 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LGL01 | Y640F | Anemia, neutropenia | 63 | F | n/a | No | CSA, MTX, ALEM | TRBV12-3, 12-4 | 84.0 | 1210 | 1 | 31 | 7 | 8 | 7 | 7 | 3 | 13 | 2 | 6 |
| LGL02 | Negative | Anemia, neutropenia | 59 | F | n/a | No | FIL, CSA, MTX | NIP | 74.7 | 463 | 2 | 24 | 7 | 15 | 3 | 7 | 1 | 3 | 2 | 5 |
| LGL03 | Negative | Anemia | 60 | M | n/a | No | CSA, MTX, DAR | TRBV19 | 85.1 | 1464 | 2 | 25 | 18 | 44 | 12 | 16 | 11 | 15 | 3 | 6 |
| LGL04 | D661Y | Anemia | 50 | M | n/a | No | CSA | TRBV20-1 | 64.3 | 1092 | 1 | 3 | 7 | 7 | 7 | 7 | 3 | 15 | 2 | 6 |
| LGL05 | D661Y | Anemia | 62 | M | n/a | No | SPL | TRBV19 | 52.7 | 1066 | 2 | 29 | 7 | 45 | 6 | 7 | 1 | 15 | 5 | 6 |
| LGL06 | Negative | Anemia | 72 | M | n/a | No | CSA, MTX, CYC, ATG | TRBV6-6 | 92.7 | 4305 | 1 | 2 | 7 | 8 | 7 | 7 | 3 | 13 | 2 | 6 |
| LGL07 | D661Y | Anemia | 54 | M | n/a | No | CYC | TRBV9 | 40.1 | 237 | 2 | 2 | 40 | 49 | 2 | 7 | 11 | 13 | 3 | 6 |
| LGL08 | Negative | Anemia | 68 | M | Parvovirus B19 | No | CSA, CYC, MTX | NIP | 65.3 | 922 | 2 | 24 | 7 | 13 | 2 | 7 | 3 | 15 | 2 | 6 |
| LGL09 | D661V | Neutropenia, thrombo-cytopenia | 81 | M | n/a | RA | MTX | TRBV9 | 71.5 | 745 | 1 | 2 | 7 | 15 | 3 | 7 | 4 | 15 | 3 | 6 |
| LGL10 | Negative | Anemia, neutropenia | 56 | F | n/a | No | CYC, MTX | TRBV12-3, 12-4 | 88.7 | 844 | 29 | 33 | 14 | 14 | 8 | 8 | 1 | 11 | 3 | 5 |
| LGL11 | Negative | Anemia | 51 | F | ANA, SSA, SSB, RNP, chromatin | RA, Sjögren | PEG, MTX | TRBV19 | 71.6 | 440 | 1 | 3 | 8 | 35 | 4 | 7 | 3 | 15 | 2 | 6 |
| Cohort ID . | STAT3 mutation . | Hematologic presentation . | AAD . | Sex . | Viral serology . | Autoimmune disease . | Therapy . | Flow VB ID . | Clone size* . | Absolute major clone size† . | HLA A . | HLA A2 . | HLA B . | HLA B2 . | HLA Cw . | HLA Cw 2 . | HLA DRB1 . | HLA DRB1 2 . | HLA DQB1 . | HLA DQB1 2 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LGL01 | Y640F | Anemia, neutropenia | 63 | F | n/a | No | CSA, MTX, ALEM | TRBV12-3, 12-4 | 84.0 | 1210 | 1 | 31 | 7 | 8 | 7 | 7 | 3 | 13 | 2 | 6 |
| LGL02 | Negative | Anemia, neutropenia | 59 | F | n/a | No | FIL, CSA, MTX | NIP | 74.7 | 463 | 2 | 24 | 7 | 15 | 3 | 7 | 1 | 3 | 2 | 5 |
| LGL03 | Negative | Anemia | 60 | M | n/a | No | CSA, MTX, DAR | TRBV19 | 85.1 | 1464 | 2 | 25 | 18 | 44 | 12 | 16 | 11 | 15 | 3 | 6 |
| LGL04 | D661Y | Anemia | 50 | M | n/a | No | CSA | TRBV20-1 | 64.3 | 1092 | 1 | 3 | 7 | 7 | 7 | 7 | 3 | 15 | 2 | 6 |
| LGL05 | D661Y | Anemia | 62 | M | n/a | No | SPL | TRBV19 | 52.7 | 1066 | 2 | 29 | 7 | 45 | 6 | 7 | 1 | 15 | 5 | 6 |
| LGL06 | Negative | Anemia | 72 | M | n/a | No | CSA, MTX, CYC, ATG | TRBV6-6 | 92.7 | 4305 | 1 | 2 | 7 | 8 | 7 | 7 | 3 | 13 | 2 | 6 |
| LGL07 | D661Y | Anemia | 54 | M | n/a | No | CYC | TRBV9 | 40.1 | 237 | 2 | 2 | 40 | 49 | 2 | 7 | 11 | 13 | 3 | 6 |
| LGL08 | Negative | Anemia | 68 | M | Parvovirus B19 | No | CSA, CYC, MTX | NIP | 65.3 | 922 | 2 | 24 | 7 | 13 | 2 | 7 | 3 | 15 | 2 | 6 |
| LGL09 | D661V | Neutropenia, thrombo-cytopenia | 81 | M | n/a | RA | MTX | TRBV9 | 71.5 | 745 | 1 | 2 | 7 | 15 | 3 | 7 | 4 | 15 | 3 | 6 |
| LGL10 | Negative | Anemia, neutropenia | 56 | F | n/a | No | CYC, MTX | TRBV12-3, 12-4 | 88.7 | 844 | 29 | 33 | 14 | 14 | 8 | 8 | 1 | 11 | 3 | 5 |
| LGL11 | Negative | Anemia | 51 | F | ANA, SSA, SSB, RNP, chromatin | RA, Sjögren | PEG, MTX | TRBV19 | 71.6 | 440 | 1 | 3 | 8 | 35 | 4 | 7 | 3 | 15 | 2 | 6 |
AAD, age at diagnosis; ALEM, alemtuzumab; ANA, anti-nuclear antibodies; ATG, antithymocyte globulin; CSA, cyclosporine; CYC, cyclophosphamide; DAR, darbepoetin; FIL, filgrastim; MTX, methotrexate; NIP, not in panel; PEG, pegfilgrastim; SPL, splenectomy; SSA, Sjögren's Syndrome A; SSB, Sjögren's Syndrome B.
Clone size: % CD8+ cells expressing a specific Vβ region by flow cytometry.
Absolute major clone size = (white blood cells) (% lymph) (% CD8) (% specific Vβ), shown in cells per microliter of blood.