Table 1 Comparison of the most commonly... | American Society of Hematology

Table 1

Comparison of the most commonly used vectors for gene therapy

Characteristics	α-Retroviral vector	γ-Retroviral vector	Foamy virus vector	rAAV	Lentivirus vector
Tropism	Dividing and nondividing cells	Dividing cells	Dividing and nondividing cells	Dividing and nondividing cells	Dividing and nondividing cells
Host genome	Neutral	Integration near regulatory elements	Integration nearly uniform	No integration	Integration in genes
Transgene expression	Stable	Stable	Stable	Stable in nondividing cells	Stable
Packaging capacity	Not defined	∼8 kb	∼9 kb	∼5 kb	∼8 kb
Advantages	Neutral, integration, pattern high-titer production	Large packaging capacity; long-term expression	Large packaging capacity; long-term expression	High production yields; low immunogenicity; long-term expression	Large packaging capacity; long-term expression
Disadvantages	None have emerged	High risk of insertional mutagenesis	Risk of insertional mutagenesis	Small packaging capacity	Risk of insertional mutagenesis

Characteristics	α-Retroviral vector	γ-Retroviral vector	Foamy virus vector	rAAV	Lentivirus vector
Tropism	Dividing and nondividing cells	Dividing cells	Dividing and nondividing cells	Dividing and nondividing cells	Dividing and nondividing cells
Host genome	Neutral	Integration near regulatory elements	Integration nearly uniform	No integration	Integration in genes
Transgene expression	Stable	Stable	Stable	Stable in nondividing cells	Stable
Packaging capacity	Not defined	∼8 kb	∼9 kb	∼5 kb	∼8 kb
Advantages	Neutral, integration, pattern high-titer production	Large packaging capacity; long-term expression	Large packaging capacity; long-term expression	High production yields; low immunogenicity; long-term expression	Large packaging capacity; long-term expression
Disadvantages	None have emerged	High risk of insertional mutagenesis	Risk of insertional mutagenesis	Small packaging capacity	Risk of insertional mutagenesis

rAAV, adeno-associated viral vector.