PAR3 peptide fragments generated by APC or thrombin proteolysis
| Fragment . | Rt . | Cleaved by . | Experimental mass . | Calculated mass . | PAR3 peptide (21-65) . | Cleavage site . |
|---|---|---|---|---|---|---|
| A | 6.3 | IIa | 1943.2 | 1943.3 | SGMENDTNNLAKPTLPIK-38 | K38 |
| B | 8.2 | IIa | 2930.7 | 2931.3 | 39-TFRGAPPNSFEEFPFSALEGWTGATIT | K38 |
| C | 8.7 | — | 4856 | 4856.6 | SGMENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWTGATIT | — |
| D | 7.0 | APC | 2347.3 | 2347.8 | SGMENDTNNLAKPTLPIKTFR-41 | R41 |
| E | 7.9 | APC | 2526.0 | 2526.9 | 42-GAPPNSFEEFPFSALEGWTGATIT | R41 |
| F | 8.7 | — | 4856 | 4856.6 | SGMENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWTGATIT | — |
| Fragment . | Rt . | Cleaved by . | Experimental mass . | Calculated mass . | PAR3 peptide (21-65) . | Cleavage site . |
|---|---|---|---|---|---|---|
| A | 6.3 | IIa | 1943.2 | 1943.3 | SGMENDTNNLAKPTLPIK-38 | K38 |
| B | 8.2 | IIa | 2930.7 | 2931.3 | 39-TFRGAPPNSFEEFPFSALEGWTGATIT | K38 |
| C | 8.7 | — | 4856 | 4856.6 | SGMENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWTGATIT | — |
| D | 7.0 | APC | 2347.3 | 2347.8 | SGMENDTNNLAKPTLPIKTFR-41 | R41 |
| E | 7.9 | APC | 2526.0 | 2526.9 | 42-GAPPNSFEEFPFSALEGWTGATIT | R41 |
| F | 8.7 | — | 4856 | 4856.6 | SGMENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWTGATIT | — |
Rt, retention time.