Sphingolipid and glycosphingolipid content of control and NPC1 lymph nodes
| Lipid content (ng/mg protein) . | SPH . | S1P . | Charged GSLs . | Neutral GSLs . |
|---|---|---|---|---|
| Control | 5.26 ± 0.17 | 0.21 ± 0.01 | 13.3 | 63.0 |
| NPC1 | 53.54 ± 4.53 | 0.57 ± 0.09 | 14.4 | 88.2 |
| Lipid content (ng/mg protein) . | SPH . | S1P . | Charged GSLs . | Neutral GSLs . |
|---|---|---|---|---|
| Control | 5.26 ± 0.17 | 0.21 ± 0.01 | 13.3 | 63.0 |
| NPC1 | 53.54 ± 4.53 | 0.57 ± 0.09 | 14.4 | 88.2 |
Liquid-chromatography tandem MS data were normalized to protein content of pooled inguinal lymph nodes; average values ± SD from 2 replicate experiments are shown (glycosphingolipid determinations were performed only once). Internal standards were used for the quantitation of lipids.
SPH, sphingosine.