Details of confirmed recurrent gene mutations in the linker HIST1H1 genes B-E in 114 FL cases
| FL case no. . | Gene . | Sequence context of mutations . | cDNA changes . | Amino acid change (protein) . | Sanger sequencing result (T/N) . | PolyPhen-2 prediction results . | SIFT prediction results . |
|---|---|---|---|---|---|---|---|
| L55 | HIST1H1B | AAGAG(C/G)TTGGTG | c.267C>C/G | 89S/R | Mut-Heter/WT | Benign (0.226) | Damaging (0) |
| L55 | HIST1H1B | CCAAGG(G/A)CACTGG | c.302G>G/A | 101G/D | Mut-Heter/WT | Probably damaging (1.0) | Damaging (0) |
| L77 | HIST1H1B | CAAGA(G/A)CTTGG | c.266G>G/A | 89S/N | Mut-Heter/WT | Possibly damaging (0.616) | Damaging (0) |
| L90 | HIST1H1B | ACCTA(A>C)AGCTG | c.641A>C | 214K->T | Mut-Homo/WT | Probably damaging (0.998) | Damaging (0.01) |
| ML91 | HIST1H1B | CTAAG(AAG)GCCAA | c.397_399Het_Del AAG | In-frame deletion | Mut-Heter/WT | ||
| FL11 | HIST1H1B | CAAAG(C/A)CAAGA | c.368C>C/A | 123A/D | Mut-Heter)/WT | Possibly damaging (0.622) | Damaging (0.01) |
| FL24 | HIST1H1B | ATTAA(G/C)CTGGG | c.252G>G/C | 84K/N | Mut-Heter/WT | Possibly damaging (0.597) | Damaging (0) |
| FL55 | HIST1H1B | CGGTG(G/C)CTACG | c.217G>G/C | 73G/A | Mut-Heter/WT | Probably damaging (1.000) | Damaging (0.02) |
| L27 | HIST1H1C | CCAAG(C/T)CCAAG | c.352C>C/T | 118P/S | Mut-Heter/WT | Possibly damaging (0.518) | Tolerated (0.55) |
| L28 | HIST1H1C | AAGTG(G/C)CTAAG | c.511G>G/C | 171A/P | Mut-Heter/WT | Probably damaging (0.987) | Tolerated (0.42) |
| L77 | HIST1H1C | CTGCC(A/T)AAAGT | c.559A>A/T | 187K/X | Mut-Heter/WT | ||
| FL54 | HIST1H1C | ATCAA(A/T)CTTG(G/C)TCTCA | c.243A>A/T; c.248G>G/C | 81K/N; 83G/A | Mut-Heter/WT | Possibly damaging (0.927) | Damaging (0) |
| FL59 | HIST1H1C | CAGAG(C>G)TCATC | c.127C>G | 43L->V | Mut-Homo/WT | Possibly damaging (0.927) | Damaging (0) |
| FL75 | HIST1H1C | TGAAAAAA(A)GCGTTG | c.192Homo_InsA | Frameshift insertion | Mut-Homo/WT | ||
| L7 | HIST1H1D | CAACA(AACA)GCCGT | c.236Het_InsAACA | Frameshift insertion | Mut-Heter/WT | ||
| L27 | HIST1H1D | AAG(G/A)TA(C/T)CGG | c.275G>G/A c.278C>C/T | 99G/D 100T/I | Mut-Heter/WT | Probably damaging (0.999) | Damaging (0) |
| L46 | HIST1H1D | ATCAA(G/C)CTTGG | c.246G>G/C | 82K/N | Mut-Heter/WT | Possibly damaging (0.774) | Damaging (0) |
| L53 | HIST1H1D | CGGTG(C/T)TTCTG | c.305C>C/T | 102A/V | Mut-Heter/WT | Probably damaging (0.995) | Damaging (0) |
| L55 | HIST1H1D | GGCGG(C/G)CAAGC | c.602C>C/G | 201A/G | Mut-Heter/WT | Benign (0.191) | Tolerated (0.65) |
| L56 | HIST1H1D | AGAAAG(C/T)GCTTG | c.197C>C/T | 66A/V | Mut-Heter/WT | Possibly damaging (0.954) | Tolerated (0.34) |
| ML64 | HIST1H1D | GCTTG(G>A)CCTCA | c.251G>A | 84G->D | Mut-Homo/WT | Probably damaging (0.98) | Damaging (0.05) |
| ML90 | HIST1H1D | CAAAG(G/A)TACTC | c.275G>G/A | 92G/D | Mut-Heter/WT | Probably damaging (0.999) | Damaging (0) |
| FL6 | HIST1H1D | GCGCC(G/C)CTACC | c.436G>G/C | 146A/P | Mut-Heter/WT | Possibly damaging (0.769) | Tolerated (0.25) |
| FL39* | HIST1H1D | CAAAGG(*)TACCGG | c.300Het_Ins* | Frameshift insertion | Mut-Heter/WT | ||
| FL52 | HIST1H1D | AACAC(C/G)TCAGC | c.542C>C/G | 181P/R | Mut-Heter/WT | Benign (0.001) | Damaging (0) |
| L23 | HIST1H1E | AGCCC(A/G)AGAAG | c.415A>A/G | 139K/E | Mut-Heter/WT | Benign (0.024) | Tolerated (0.06) |
| L24 | HIST1H1E | CCAAG(C/G)CAAAA | c.541C>C/G | 181P/A | Mut-Heter/WT | Benign (0.096) | Tolerated (1) |
| L27 | HIST1H1E | CTGCA(G/A)CTGCT | c.490G>G/A | 164A/T | Mut-Heter/WT | Possibly damaging (0.917) | Tolerated (0.07) |
| L64 | HIST1H1E | GCCTA(A/G)GGCTA | c.356A>A/G | 120A/T | Mut-Heter/WT | Benign (0) | Tolerated (1) |
| L114 | HIST1H1E | GGTTC(C)TTCAA | c.312Het_DelC | Frameshift insertion | Mut-Heter/WT | ||
| ML47 | HIST1H1E | CTAAA(G/A)CTGTT | c.139G>G/A | 47A/T | Mut-Heter/WT | Possibly damaging (0.597) | Tolerated (0.08) |
| ML90 | HIST1H1E | GTCGG(G/A)TTCCT; | c.308G>G/A | 103G/D | Mut-Heter/WT | Probably damaging (1.0) | Damaging (0) |
| ML90 | HIST1H1E | AGCCA(G/A)CAGGA | c.394G>G/A | 132A/T | Mut-Heter/WT | Benign (0) | Tolerated (0.56) |
| FL7 | HIST1H1E | CATTA(C/G)TAAAG | c.134C>C/G | 45T/S | Mut-Heter/WT | Possibly damaging (0.795) | Damaging (0.04) |
| FL29 | HIST1H1E | GCGGC(G/A)TATCT | c.169G>G/A | 57V/I | Mut-Heter/WT | Possibly damaging (0.951) | Tolerated (0.15) |
| FL34 | HIST1H1E | AGACC(C/T)CAAAG | c.463C>C/T | 155P/S | Mut-Heter//WT | Possibly damaging (0.799) | Tolerated (0.46) |
| FL39 | HIST1H1E | GGGTT(G/T)CTTCA | c.311G>G/T | 104S/F | Mut-Heter/WT | Probably damaging (0.975) | Damaging (0) |
| FL39 | HIST1H1E | TGGAG(C/T)CAAAA | c.500C>C/T | 167A/V | Mut-Heter/WT | Benign (0.209) | Tolerated (0.24) |
| FL42 | HIST1H1E | CCAAG(C/T)CAAAA | c.541C>C/T | 181P/S | Mut-Heter/WT | Possibly damaging (0.798) | Tolerated (0.66) |
| FL61 | HIST1H1E | CTAAA(A/T)AGGCA | c.364A>A/T | 122K/X | Mut-Heter/WT |
| FL case no. . | Gene . | Sequence context of mutations . | cDNA changes . | Amino acid change (protein) . | Sanger sequencing result (T/N) . | PolyPhen-2 prediction results . | SIFT prediction results . |
|---|---|---|---|---|---|---|---|
| L55 | HIST1H1B | AAGAG(C/G)TTGGTG | c.267C>C/G | 89S/R | Mut-Heter/WT | Benign (0.226) | Damaging (0) |
| L55 | HIST1H1B | CCAAGG(G/A)CACTGG | c.302G>G/A | 101G/D | Mut-Heter/WT | Probably damaging (1.0) | Damaging (0) |
| L77 | HIST1H1B | CAAGA(G/A)CTTGG | c.266G>G/A | 89S/N | Mut-Heter/WT | Possibly damaging (0.616) | Damaging (0) |
| L90 | HIST1H1B | ACCTA(A>C)AGCTG | c.641A>C | 214K->T | Mut-Homo/WT | Probably damaging (0.998) | Damaging (0.01) |
| ML91 | HIST1H1B | CTAAG(AAG)GCCAA | c.397_399Het_Del AAG | In-frame deletion | Mut-Heter/WT | ||
| FL11 | HIST1H1B | CAAAG(C/A)CAAGA | c.368C>C/A | 123A/D | Mut-Heter)/WT | Possibly damaging (0.622) | Damaging (0.01) |
| FL24 | HIST1H1B | ATTAA(G/C)CTGGG | c.252G>G/C | 84K/N | Mut-Heter/WT | Possibly damaging (0.597) | Damaging (0) |
| FL55 | HIST1H1B | CGGTG(G/C)CTACG | c.217G>G/C | 73G/A | Mut-Heter/WT | Probably damaging (1.000) | Damaging (0.02) |
| L27 | HIST1H1C | CCAAG(C/T)CCAAG | c.352C>C/T | 118P/S | Mut-Heter/WT | Possibly damaging (0.518) | Tolerated (0.55) |
| L28 | HIST1H1C | AAGTG(G/C)CTAAG | c.511G>G/C | 171A/P | Mut-Heter/WT | Probably damaging (0.987) | Tolerated (0.42) |
| L77 | HIST1H1C | CTGCC(A/T)AAAGT | c.559A>A/T | 187K/X | Mut-Heter/WT | ||
| FL54 | HIST1H1C | ATCAA(A/T)CTTG(G/C)TCTCA | c.243A>A/T; c.248G>G/C | 81K/N; 83G/A | Mut-Heter/WT | Possibly damaging (0.927) | Damaging (0) |
| FL59 | HIST1H1C | CAGAG(C>G)TCATC | c.127C>G | 43L->V | Mut-Homo/WT | Possibly damaging (0.927) | Damaging (0) |
| FL75 | HIST1H1C | TGAAAAAA(A)GCGTTG | c.192Homo_InsA | Frameshift insertion | Mut-Homo/WT | ||
| L7 | HIST1H1D | CAACA(AACA)GCCGT | c.236Het_InsAACA | Frameshift insertion | Mut-Heter/WT | ||
| L27 | HIST1H1D | AAG(G/A)TA(C/T)CGG | c.275G>G/A c.278C>C/T | 99G/D 100T/I | Mut-Heter/WT | Probably damaging (0.999) | Damaging (0) |
| L46 | HIST1H1D | ATCAA(G/C)CTTGG | c.246G>G/C | 82K/N | Mut-Heter/WT | Possibly damaging (0.774) | Damaging (0) |
| L53 | HIST1H1D | CGGTG(C/T)TTCTG | c.305C>C/T | 102A/V | Mut-Heter/WT | Probably damaging (0.995) | Damaging (0) |
| L55 | HIST1H1D | GGCGG(C/G)CAAGC | c.602C>C/G | 201A/G | Mut-Heter/WT | Benign (0.191) | Tolerated (0.65) |
| L56 | HIST1H1D | AGAAAG(C/T)GCTTG | c.197C>C/T | 66A/V | Mut-Heter/WT | Possibly damaging (0.954) | Tolerated (0.34) |
| ML64 | HIST1H1D | GCTTG(G>A)CCTCA | c.251G>A | 84G->D | Mut-Homo/WT | Probably damaging (0.98) | Damaging (0.05) |
| ML90 | HIST1H1D | CAAAG(G/A)TACTC | c.275G>G/A | 92G/D | Mut-Heter/WT | Probably damaging (0.999) | Damaging (0) |
| FL6 | HIST1H1D | GCGCC(G/C)CTACC | c.436G>G/C | 146A/P | Mut-Heter/WT | Possibly damaging (0.769) | Tolerated (0.25) |
| FL39* | HIST1H1D | CAAAGG(*)TACCGG | c.300Het_Ins* | Frameshift insertion | Mut-Heter/WT | ||
| FL52 | HIST1H1D | AACAC(C/G)TCAGC | c.542C>C/G | 181P/R | Mut-Heter/WT | Benign (0.001) | Damaging (0) |
| L23 | HIST1H1E | AGCCC(A/G)AGAAG | c.415A>A/G | 139K/E | Mut-Heter/WT | Benign (0.024) | Tolerated (0.06) |
| L24 | HIST1H1E | CCAAG(C/G)CAAAA | c.541C>C/G | 181P/A | Mut-Heter/WT | Benign (0.096) | Tolerated (1) |
| L27 | HIST1H1E | CTGCA(G/A)CTGCT | c.490G>G/A | 164A/T | Mut-Heter/WT | Possibly damaging (0.917) | Tolerated (0.07) |
| L64 | HIST1H1E | GCCTA(A/G)GGCTA | c.356A>A/G | 120A/T | Mut-Heter/WT | Benign (0) | Tolerated (1) |
| L114 | HIST1H1E | GGTTC(C)TTCAA | c.312Het_DelC | Frameshift insertion | Mut-Heter/WT | ||
| ML47 | HIST1H1E | CTAAA(G/A)CTGTT | c.139G>G/A | 47A/T | Mut-Heter/WT | Possibly damaging (0.597) | Tolerated (0.08) |
| ML90 | HIST1H1E | GTCGG(G/A)TTCCT; | c.308G>G/A | 103G/D | Mut-Heter/WT | Probably damaging (1.0) | Damaging (0) |
| ML90 | HIST1H1E | AGCCA(G/A)CAGGA | c.394G>G/A | 132A/T | Mut-Heter/WT | Benign (0) | Tolerated (0.56) |
| FL7 | HIST1H1E | CATTA(C/G)TAAAG | c.134C>C/G | 45T/S | Mut-Heter/WT | Possibly damaging (0.795) | Damaging (0.04) |
| FL29 | HIST1H1E | GCGGC(G/A)TATCT | c.169G>G/A | 57V/I | Mut-Heter/WT | Possibly damaging (0.951) | Tolerated (0.15) |
| FL34 | HIST1H1E | AGACC(C/T)CAAAG | c.463C>C/T | 155P/S | Mut-Heter//WT | Possibly damaging (0.799) | Tolerated (0.46) |
| FL39 | HIST1H1E | GGGTT(G/T)CTTCA | c.311G>G/T | 104S/F | Mut-Heter/WT | Probably damaging (0.975) | Damaging (0) |
| FL39 | HIST1H1E | TGGAG(C/T)CAAAA | c.500C>C/T | 167A/V | Mut-Heter/WT | Benign (0.209) | Tolerated (0.24) |
| FL42 | HIST1H1E | CCAAG(C/T)CAAAA | c.541C>C/T | 181P/S | Mut-Heter/WT | Possibly damaging (0.798) | Tolerated (0.66) |
| FL61 | HIST1H1E | CTAAA(A/T)AGGCA | c.364A>A/T | 122K/X | Mut-Heter/WT |
Boldface text indicates the nucleotide change from WT (first) to mutated (second).
cDNA, complementary DNA; T/N, tumor/normal; WT, wild-type.
Full insert sequence: (AACAGCCGGTATCAAGCTTGGCCTCAAGAGCTTGGTGAGCAAAGGTACTCTGGTGCAGACCAAAGG).