Characteristics of EC and BFU-E derived from transduced CD34+ cells cultured in vitro
| Experiment . | Avg VCN liquid EC . | Frequency vector +ve BFU-E (%) . | β/α* Expression BFU-E (%) . |
|---|---|---|---|
| Preinfusion (in vitro) | |||
| 1 | 0.38 | 21.2 | ND |
| 2 | 1.40 | 23.0 | 50 ± 13.7 |
| 3 | 0.74 | 30.4 | ND |
| 4 | 0.63 | 34.8 | ND |
| 5 | 0.58 | 30.0 | ND |
| Experiment . | Avg VCN liquid EC . | Frequency vector +ve BFU-E (%) . | β/α* Expression BFU-E (%) . |
|---|---|---|---|
| Preinfusion (in vitro) | |||
| 1 | 0.38 | 21.2 | ND |
| 2 | 1.40 | 23.0 | 50 ± 13.7 |
| 3 | 0.74 | 30.4 | ND |
| 4 | 0.63 | 34.8 | ND |
| 5 | 0.58 | 30.0 | ND |
The average VCN in liquid EC and the frequency of BFU-E colonies positive for the vector were determined in 5 independent experiments. β/α* expression was measured in pooled BFU-Es: untransduced pool, n = 30; transduced with TNS9.3.55 n = 30; average VCN = 1.6. ND, not done.