Table 2 In vitro assays of Haemonetics,... | American Society of Hematology

Table 2

In vitro assays of Haemonetics, Spectra, and Trima apheresis platelets stored in PAS

N	Storage time (days)	Donor’s platelet count (μL)	Unit volume (mL)	Total platelet count (×10¹¹)		PAS (%)	Glucose (mgm/dL)*		MPV	Morphology score	Annexin V binding (%)	ESC (%)	HSR (%)	pH
N	Storage time (days)	Donor’s platelet count (μL)	Unit volume (mL)	Day 1	Test day	PAS (%)	Day 1	Test day	MPV	Morphology score	Annexin V binding (%)	ESC (%)	HSR (%)	pH
Haemonetics apheresis platelets
6	5	240 000 ± 29 000	189 ± 23	ND	1.76 ± 0.46	78 ± 7	ND	ND	ND	229 ± 14	16 ± 2	26 ± 5	77 ± 9	7.1 ± 0.1
9	7	232 000 ± 51 000	215 ± 23	ND	2.02 ± 0.43	69 ± 12	ND	ND	ND	250 ± 26	17 ± 7	17 ± 3	70 ± 14	7.1 ± 0.6
4	9	288 000 ± 39 000	217 ± 53	2.65 ± 0.70	2.74 ± 0.69	79 ± 3	77 ± 10	5.5 ± 6.1	ND	325 ± 35	23 ± 5	18 ± 3	87 ± 7	7.4 ± 0.2
10	13	234 000 ± 31 000	258 ± 28	2.59 ± 0.58	2.51 ± 0.53	81 ± 1	74 ± 7	7.0 ± 15	6.9 ± 1.1	265 ± 48	26 ± 14	13 ± 5	94 ± 7	7.4 ± 0.2
10	14	215 000 ± 45 000	263 ± 47	2.22 ± 0.53	2.00 ± 0.47	82 ± 1	74 ± 1	ND	6.7 ± 0.7	252 ± 52	34 ± 10	11 ± 3	66 ± 19	7.4 ± 0.2
3	15	245 000 ± 43 000	200 ± 48	2.33 ± 0.92	2.04 ± 0.58	80 ± 2	76 ± 2	0.3 ± 0.6	7.5 ± 0.8	220 ± 25	33 ± 6	9 ± 5	67 ± 14	7.5 ± 0.1
8	15†	254 000 ± 73 000	230 ± 56	2.26 ± 0.63	1.83 ± 0.72	76 ± 4	81 ± 9	1.1 ± 0.8	8.0 ± 0.5	245 ± 46	40 ± 15	4 ± 3	25 ± 11	7.5 ± 0.3
3	17	269 000 ± 67 000	218 ± 20	2.88 ± 0.65	2.56 ± 0.84	82 ± 3	ND	ND	7.4 ± 0.7	197 ± 10	35 ± 11	10 ± 6	61 ± 15	7.7 ± 0.1
3	18	242 000 ± 56 000	252 ± 78	2.95 ± 0.35	2.27 ± 0.75	78 ± 4	72 ± 3	0.3 ± 0.6	7.3 ± 0.3	225 ± 56	32 ± 21	9 ± 6	29 ± 14	7.5 ± 0.2
Spectra apheresis platelets
3	≤1	194 000 ± 1,000	275 ± 24	2.36 ± 0.2	ND	80 ± 1	ND	68 ± 6	ND	368 ± 6	7 ± 2	16 ± 1	64 ± 13	7.2 ± 0.2
5	5	280 000 ± 68 000	250 ± 22	ND	2.73 ± 0.46	78 ± 2	ND	49 ± 11	ND	298 ± 50	10 ± 3	13 ± 5	63 ± 9	ND
15	7	242 000 ± 58 000	276 ± 46	2.38 ± 0.12	2.34 ± 0.69	80 ± 5	ND	31 ± 30	ND	240 ± 107	29 ± 26	11 ± 4	54 ± 15	6.7 ± 0.5
5	8	252 000 ± 128 000	268 ± 27	2.17 ± 1.0	2.21 ± 0.88	80 ± 1	ND	16 ± 16	ND	296 ± 58	28 ± 6	9 ± 3	50 ± 16	7.1 ± 0.6
5	9	225 000 ± 43 000	261 ± 67	ND	1.80 ± 1.1	80 ± 1	ND	3 ± 7	ND	233 ± 46	61 ± 30	ND	31 ± 24	6.6 ± 0.7
6	13	228 000 ± 8,000	268 ± 11	2.16 ± 0.10	1.81 ± 0.1	84 ± 1	ND	ND	8 ± 0.7	240 ± 14	52 ± 13	8 ± 2	36 ± 4	7.1 ± 0.2
6	13‡	228 000 ± 8,000	288 ± 13	2.14 ± 0.12	2.09 ± 0.1	84 ± 1	ND	ND	8 ± 0.8	251 ± 17	44 ± 15	7 ± 1	45 ± 4	6.9 ± 0.2
Trima apheresis platelets
9	7	261 000 ± 81 000	297 ± 11	2.37 ± 0.62	2.34 ± 0.60	83 ± 1	ND	29 ± 15	7 ± 0.7	258 ± 38	14 ± 6	13 ± 3	38 ± 28	6.8 ± 0.2
8	9	300 000 ± 89 000	311 ± 31	2.16 ± 0.56	2.10 ± 0.61	83 ± 4	ND	11 ± 13	7 ± 0.6	193 ± 186	26 ± 15	9 ± 5	36 ± 23	6.5 ± 0.3
10	9‡	257 000 ± 40 000	298 ± 12	2.49 ± 0.62	2.38 ± 0.56	82 ± 2	ND	19 ± 16	7 ± 0.4	302 ± 19	16 ± 6	12 ± 2	43 ± 11	6.8 ± 0.2

N	Storage time (days)	Donor’s platelet count (μL)	Unit volume (mL)	Total platelet count (×10¹¹)		PAS (%)	Glucose (mgm/dL)*		MPV	Morphology score	Annexin V binding (%)	ESC (%)	HSR (%)	pH
N	Storage time (days)	Donor’s platelet count (μL)	Unit volume (mL)	Day 1	Test day	PAS (%)	Day 1	Test day	MPV	Morphology score	Annexin V binding (%)	ESC (%)	HSR (%)	pH
Haemonetics apheresis platelets
6	5	240 000 ± 29 000	189 ± 23	ND	1.76 ± 0.46	78 ± 7	ND	ND	ND	229 ± 14	16 ± 2	26 ± 5	77 ± 9	7.1 ± 0.1
9	7	232 000 ± 51 000	215 ± 23	ND	2.02 ± 0.43	69 ± 12	ND	ND	ND	250 ± 26	17 ± 7	17 ± 3	70 ± 14	7.1 ± 0.6
4	9	288 000 ± 39 000	217 ± 53	2.65 ± 0.70	2.74 ± 0.69	79 ± 3	77 ± 10	5.5 ± 6.1	ND	325 ± 35	23 ± 5	18 ± 3	87 ± 7	7.4 ± 0.2
10	13	234 000 ± 31 000	258 ± 28	2.59 ± 0.58	2.51 ± 0.53	81 ± 1	74 ± 7	7.0 ± 15	6.9 ± 1.1	265 ± 48	26 ± 14	13 ± 5	94 ± 7	7.4 ± 0.2
10	14	215 000 ± 45 000	263 ± 47	2.22 ± 0.53	2.00 ± 0.47	82 ± 1	74 ± 1	ND	6.7 ± 0.7	252 ± 52	34 ± 10	11 ± 3	66 ± 19	7.4 ± 0.2
3	15	245 000 ± 43 000	200 ± 48	2.33 ± 0.92	2.04 ± 0.58	80 ± 2	76 ± 2	0.3 ± 0.6	7.5 ± 0.8	220 ± 25	33 ± 6	9 ± 5	67 ± 14	7.5 ± 0.1
8	15†	254 000 ± 73 000	230 ± 56	2.26 ± 0.63	1.83 ± 0.72	76 ± 4	81 ± 9	1.1 ± 0.8	8.0 ± 0.5	245 ± 46	40 ± 15	4 ± 3	25 ± 11	7.5 ± 0.3
3	17	269 000 ± 67 000	218 ± 20	2.88 ± 0.65	2.56 ± 0.84	82 ± 3	ND	ND	7.4 ± 0.7	197 ± 10	35 ± 11	10 ± 6	61 ± 15	7.7 ± 0.1
3	18	242 000 ± 56 000	252 ± 78	2.95 ± 0.35	2.27 ± 0.75	78 ± 4	72 ± 3	0.3 ± 0.6	7.3 ± 0.3	225 ± 56	32 ± 21	9 ± 6	29 ± 14	7.5 ± 0.2
Spectra apheresis platelets
3	≤1	194 000 ± 1,000	275 ± 24	2.36 ± 0.2	ND	80 ± 1	ND	68 ± 6	ND	368 ± 6	7 ± 2	16 ± 1	64 ± 13	7.2 ± 0.2
5	5	280 000 ± 68 000	250 ± 22	ND	2.73 ± 0.46	78 ± 2	ND	49 ± 11	ND	298 ± 50	10 ± 3	13 ± 5	63 ± 9	ND
15	7	242 000 ± 58 000	276 ± 46	2.38 ± 0.12	2.34 ± 0.69	80 ± 5	ND	31 ± 30	ND	240 ± 107	29 ± 26	11 ± 4	54 ± 15	6.7 ± 0.5
5	8	252 000 ± 128 000	268 ± 27	2.17 ± 1.0	2.21 ± 0.88	80 ± 1	ND	16 ± 16	ND	296 ± 58	28 ± 6	9 ± 3	50 ± 16	7.1 ± 0.6
5	9	225 000 ± 43 000	261 ± 67	ND	1.80 ± 1.1	80 ± 1	ND	3 ± 7	ND	233 ± 46	61 ± 30	ND	31 ± 24	6.6 ± 0.7
6	13	228 000 ± 8,000	268 ± 11	2.16 ± 0.10	1.81 ± 0.1	84 ± 1	ND	ND	8 ± 0.7	240 ± 14	52 ± 13	8 ± 2	36 ± 4	7.1 ± 0.2
6	13‡	228 000 ± 8,000	288 ± 13	2.14 ± 0.12	2.09 ± 0.1	84 ± 1	ND	ND	8 ± 0.8	251 ± 17	44 ± 15	7 ± 1	45 ± 4	6.9 ± 0.2
Trima apheresis platelets
9	7	261 000 ± 81 000	297 ± 11	2.37 ± 0.62	2.34 ± 0.60	83 ± 1	ND	29 ± 15	7 ± 0.7	258 ± 38	14 ± 6	13 ± 3	38 ± 28	6.8 ± 0.2
8	9	300 000 ± 89 000	311 ± 31	2.16 ± 0.56	2.10 ± 0.61	83 ± 4	ND	11 ± 13	7 ± 0.6	193 ± 186	26 ± 15	9 ± 5	36 ± 23	6.5 ± 0.3
10	9‡	257 000 ± 40 000	298 ± 12	2.49 ± 0.62	2.38 ± 0.56	82 ± 2	ND	19 ± 16	7 ± 0.4	302 ± 19	16 ± 6	12 ± 2	43 ± 11	6.8 ± 0.2

Data are given as the average ± 1 standard deviation. ND, not done.

Glucose levels can be accurately measured to 0 mgm/dL.

†

These platelets were stored in Haemonetics CPP bags vs all other studies that were stored in Haemonetics CLX bags.

‡

Platelets stored in Haemonetics CLX bags.

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