Generation of Lman1 conditional knockout mice
| Crosses . | Genotype distribution at 3 wk . | P value (χ2) . | |||
|---|---|---|---|---|---|
| Lman1fl/+, Cre− . | Lman1fl/+, Cre+ . | Lman1fl/fl, Cre− . | Lman1fl/fl, Cre+ . | ||
| Expected % | 25% | 25% | 25% | 25% | |
| Lman1fl/fl × Lman1fl/+, Alb-Cre+ | 24% (17) | 25% (18) | 19% (14) | 32% (23) | .5 (NS) |
| Lman1fl/fl × Lman1fl/+, Tek-Cre+ | 19% (16) | 28% (23) | 26.5% (22) | 26.5% (22) | .7 (NS) |
| Crosses . | Genotype distribution at 3 wk . | P value (χ2) . | |||
|---|---|---|---|---|---|
| Lman1fl/+, Cre− . | Lman1fl/+, Cre+ . | Lman1fl/fl, Cre− . | Lman1fl/fl, Cre+ . | ||
| Expected % | 25% | 25% | 25% | 25% | |
| Lman1fl/fl × Lman1fl/+, Alb-Cre+ | 24% (17) | 25% (18) | 19% (14) | 32% (23) | .5 (NS) |
| Lman1fl/fl × Lman1fl/+, Tek-Cre+ | 19% (16) | 28% (23) | 26.5% (22) | 26.5% (22) | .7 (NS) |
The χ2 test (degrees of freedom = 3) was based upon an expected genotype ratio of 3:1, with Lman1fl/fl/Cre+ mice expected to represent 25% of the offspring from each mating and all other genotypes cumulatively accounting for 75% of offspring.
NS, not significant.