Table 3

Gene expression average fold-change in the whole-blood mRNA relative to total liver lysate (input)

GeneExpressionFold-change ± SEMRangeP value
Cdh5 Endothelial 0.19 ± 0.02 0.17-0.22 <3.5 × 10−2 
Tek Endothelial 0.04 ± 0.01 0.034-0.046 <2.0 × 10−3 
Vcam1 Endothelial 0.001 ± 0.004 0.001-0.009 <5.6 × 10−3 
Vwf Endothelial/megakaryocyte 113.40 ± 7.09 106.6-120.7 <3.0 × 10−6 
F5 Hepatic/megakaryocyte 1.31 ± 0.06 1.25-1.36 >.3 
F8 0.01 ± 0.01 0.007-0.012 <1.0 × 10−4 
GeneExpressionFold-change ± SEMRangeP value
Cdh5 Endothelial 0.19 ± 0.02 0.17-0.22 <3.5 × 10−2 
Tek Endothelial 0.04 ± 0.01 0.034-0.046 <2.0 × 10−3 
Vcam1 Endothelial 0.001 ± 0.004 0.001-0.009 <5.6 × 10−3 
Vwf Endothelial/megakaryocyte 113.40 ± 7.09 106.6-120.7 <3.0 × 10−6 
F5 Hepatic/megakaryocyte 1.31 ± 0.06 1.25-1.36 >.3 
F8 0.01 ± 0.01 0.007-0.012 <1.0 × 10−4 

To evaluate the potential contribution of blood contamination to transcript levels of endothelial-specific genes, the tissue lysates of liver samples were set as a reference and the ΔCt values of individual blood samples (n = 4) were related to the mean ΔCt of the reference group. SEM, standard error of the mean.

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