Nonrestrictive linear amplification-mediated insertion sites of 2bF8 transgene determined by nrLAM-PCR
| No. . | Chromosome . | Direction of transgene . | Gene . |
|---|---|---|---|
| 1 | 1 | ↑ | ↓ DDI2 (DNA damage inducible protein 2) intron 1 |
| 2 | 1 | ↑ | ↑ ZSWIM5 (zinc finger, SWIM-domain-containing 5) intron 1 |
| 3 | 1 | ↑ | ↓ AHS1L (probable histone-lysine N-methyltransferase ASH1L) intron 14 |
| 4 | 3 | ↑ | ↓ CLASP2 (cytoplasmic linker-associated protein 2) intron 12 |
| 5 | 6 | ↑ | Intergenic (located downstream of hypothetical protein LOC100288169 and upstream of DUSP22 [dual-specificity phosphatase 22]) |
| 6 | 7 | ↓ | ↑ DGKB (diacylglyceral kinase β isoform 1, 2) intron 2 |
| 7 | 7 | ↑ | ↑ GTF2IRD2 (general transcription factor II-I repeat domain-containing protein 2A) intron 2 |
| 8 | 7 | ↓ | ↓ PILRB (paired immunoglobulin-like type 2 receptor β precursor) intron 3 |
| 9 | 8 | ↑ | Intergenic (located downstream of UNC5D [netrin receptor UNC5D precursor] and upstream of KCNUI [potassium channel subfamily U member]) |
| 10 | 11 | ↓ | ↓ RBM14-RBM41 (RBM14-RBM4 protein isoform 1, 2) intron 1 |
| 11 | 11 | ↓ | ↑ ARRB1 (β-arrestin-1 isoform A and B) intron 1 |
| 12 | 11 | ↓ | ↑ BSCL2 (Bernadinelli-Seip congenital lipidystrophy 2 [Seipin]) intron 5 |
| 13 | 11 | ↓ | Intergenic (located downstream of LTBP3 [latent transforming growth factor β binding protein 3] and upstream of SSSCA1 [Sjogren syndrome/scleroderma autoantigen 1]) |
| 14 | 12 | ↓ | Intergenic (located downstream of ATP2B1 [plasma membrane calcium-transporting ATPase 1 isoform 1a, 1b] and upstream of C12orf12 [hypothetical protein LOC196477]) |
| 15 | 14 | ↑ | ↓ SOCS4 (suppressor of cytokine signaling 4) exon 4 in 3′UT |
| 16 | 14 | ↓ | ↑ GPR132 (G protein-coupled receptor 132) intron 1 |
| 17 | 16 | ↑ | ↑ CREBBP (Creb-binding protein) exon 30 |
| 18 | 16 | ↑ | ↑ KIAA0430 (Limkain b-1 isoform 1, 2, 3) intron 6 |
| 19 | 17 | ↓ | ↓ SUZ12 (suppressor of zeste 12 homolog [Drosophila]) intron 15 |
| No. . | Chromosome . | Direction of transgene . | Gene . |
|---|---|---|---|
| 1 | 1 | ↑ | ↓ DDI2 (DNA damage inducible protein 2) intron 1 |
| 2 | 1 | ↑ | ↑ ZSWIM5 (zinc finger, SWIM-domain-containing 5) intron 1 |
| 3 | 1 | ↑ | ↓ AHS1L (probable histone-lysine N-methyltransferase ASH1L) intron 14 |
| 4 | 3 | ↑ | ↓ CLASP2 (cytoplasmic linker-associated protein 2) intron 12 |
| 5 | 6 | ↑ | Intergenic (located downstream of hypothetical protein LOC100288169 and upstream of DUSP22 [dual-specificity phosphatase 22]) |
| 6 | 7 | ↓ | ↑ DGKB (diacylglyceral kinase β isoform 1, 2) intron 2 |
| 7 | 7 | ↑ | ↑ GTF2IRD2 (general transcription factor II-I repeat domain-containing protein 2A) intron 2 |
| 8 | 7 | ↓ | ↓ PILRB (paired immunoglobulin-like type 2 receptor β precursor) intron 3 |
| 9 | 8 | ↑ | Intergenic (located downstream of UNC5D [netrin receptor UNC5D precursor] and upstream of KCNUI [potassium channel subfamily U member]) |
| 10 | 11 | ↓ | ↓ RBM14-RBM41 (RBM14-RBM4 protein isoform 1, 2) intron 1 |
| 11 | 11 | ↓ | ↑ ARRB1 (β-arrestin-1 isoform A and B) intron 1 |
| 12 | 11 | ↓ | ↑ BSCL2 (Bernadinelli-Seip congenital lipidystrophy 2 [Seipin]) intron 5 |
| 13 | 11 | ↓ | Intergenic (located downstream of LTBP3 [latent transforming growth factor β binding protein 3] and upstream of SSSCA1 [Sjogren syndrome/scleroderma autoantigen 1]) |
| 14 | 12 | ↓ | Intergenic (located downstream of ATP2B1 [plasma membrane calcium-transporting ATPase 1 isoform 1a, 1b] and upstream of C12orf12 [hypothetical protein LOC196477]) |
| 15 | 14 | ↑ | ↓ SOCS4 (suppressor of cytokine signaling 4) exon 4 in 3′UT |
| 16 | 14 | ↓ | ↑ GPR132 (G protein-coupled receptor 132) intron 1 |
| 17 | 16 | ↑ | ↑ CREBBP (Creb-binding protein) exon 30 |
| 18 | 16 | ↑ | ↑ KIAA0430 (Limkain b-1 isoform 1, 2, 3) intron 6 |
| 19 | 17 | ↓ | ↓ SUZ12 (suppressor of zeste 12 homolog [Drosophila]) intron 15 |
Arrows indicate the direction of the transgene or gene relative to the standard chromosomal numbering. The insertion sites listed in this table were from 5 NSG recipients.