Table 1
Theoretical mass-to-charge ratios (m/z) of human IgG1-Fc glycopeptides detected by nano-liquid chromatography–electrospray ionization-mass spectrometry
| Glycan species* | Structural scheme† | Theoretical glycopeptide m/z |
|---|---|---|
| G0 |  | 830.00 |
| G0F |  | 878.69 |
| G1 |  | 884.02 |
| G1F |  | 932.71 |
| G2 |  | 938.04 |
| G0FN |  | 946.38 |
| G2F |  | 986.72 |
| G1FN |  | 1000.40 |
| G2FN |  | 1054.42 |
| G1FS |  | 1029.74 |
| G2S |  | 1035.07 |
| G2FS |  | 1083.75 |
| G1FNS |  | 1097.43 |
| G2FNS |  | 1151.45 |
| Glycan species* | Structural scheme† | Theoretical glycopeptide m/z |
|---|---|---|
| G0 | | 830.00 |
| G0F | | 878.69 |
| G1 | | 884.02 |
| G1F | | 932.71 |
| G2 | | 938.04 |
| G0FN | | 946.38 |
| G2F | | 986.72 |
| G1FN | | 1000.40 |
| G2FN | | 1054.42 |
| G1FS | | 1029.74 |
| G2S | | 1035.07 |
| G2FS | | 1083.75 |
| G1FNS | | 1097.43 |
| G2FNS | | 1151.45 |
The observed tryptic IgG1-Fc glycopeptides have a common peptide moiety (p) of E293EQYNSTYR301 (Swiss-Prot entry number P01857). The m/z values of the triple protonated species are given.
*
Glycan structural features are given in terms of number of galactoses (G0, G1, G2), fucose (F), bisecting N-acetylglucosamine (N), and N-acetylneuraminic acid (sialic acid) (S).
†
Blue square indicates N-acetylglucosamine; green circle, mannose; yellow circle, galactose; purple diamond, N-acetylneuraminic acid (sialic acid); and red triangle, fucose.