Characteristics of the 22 pre-B ALL patients/leukemia samples investigated in engraftment studies
| UPN . | Age (years) . | Gender . | Disease status . | Sample type . | Cytogenetics . | Major cytogenetic group/risk status . |
|---|---|---|---|---|---|---|
| 1 | 59.4 | F | Diagnosis | PB | Failed* | t(9;22)/high |
| 2 | 44.5 | F | Diagnosis | PB | 45,XX,-7,t(9;22)(q34;q11)[4] | t(9;22)/high |
| 3 | 19.5 | M | Diagnosis | BM | Normal | Normal/standard |
| 4 | 26.1 | M | Diagnosis | BM | 55-56, XY,+X,+4,+6,+11,+12,+14,+17,+18,+21,+21[cp4]/46,XY[4] | High hyperdiploidy/ standard |
| 5 | 46.4 | F | Diagnosis | BM | 46,XX,t(9;22)(q34;q11)[1]/46,idem,der(10)t(10;20)(q22;p12)?del(10)(q22q34),der(20)t(10;20)[7]/46,XX[3] | t(9;22)/high |
| 6 | 33.6 | M | Diagnosis | PB | 46,XY,+1,dic(1;9)(p1;p1)[8]/46,XY[2] | Other/standard |
| 7 | 42.6 | M | Diagnosis | PB | 46,XY,t(4;11)(q21;q23)[10] | t(4;11)/high |
| 8 | 39.7 | M | Diagnosis | BM | 46,XY,der(9)add(9)(p11)add(9)(q34)[16]/46,XY[4] | Other/standard |
| 9 | 47.7 | M | Diagnosis | BM | 46,XY[20] | Normal/standard |
| 10 | 42.8 | F | Diagnosis | PB | 46,XX,t(2;11)(p11;p11),t(4;11)(q21;q23),i(7)(q10),add(22)(q13)[18]/46,XX[2] | t(4;11)/high |
| 11 | 39.8 | M | Diagnosis | PB | 59-62,XXYY,-1,-2,-3,-7,+8,add(9)(p1),der(9)t(9;22)(q34;q11)x2,-10,-12,-14,-15,-19,-20,+21,-22,-22,+2mar[cp6] | t(9;22)/high |
| 12 | 22.7 | M | Diagnosis | BM | 47,XY,+21c[20] | t(12;21)/standard |
| 13 | 50.4 | M | Diagnosis | BM | 46,XY,t(9;22)(q34;q11)[3]/46,XY[1] | t(9;22)/high |
| 14 | 48.2 | M | Diagnosis | PB | 46,XY,t(4;11)(q21;q23)[19]/46,XY[1] | t(4;11)/high |
| 15 | 49.3 | F | Diagnosis | PB | 46,XX,del(5)(q11q1?3),add(9)(p2?4)x2,der(19)t(1;19)(q23;p13)[35]/46,XX[5] | t(1;19)/standard |
| 16 | 42.7 | M | Diagnosis | PB | 46,XY,der(15)t(1;15)(q2;p1),der(19)t(1;19)(q23;p13)[11]† | t(1;19)/standard |
| 17 | 50.2 | F | Diagnosis | BM | Normal | Normal/standard |
| 18 | 52.4 | M | Diagnosis | BM | 45∼46,XX,inv(1)(p2q2),-4,add(4)(q2),del(6)(q1q2),add(8)(q24),add(9)(p2),add(9)(q2),-11,+2mar[cp10] | Complex/high |
| 19 | 48.7 | M | Diagnosis | BM | 46,XX,t(9;22)(q34;q11.2)[16]/46,XX[4]. | t(9;22)/high |
| 20 | 32.0 | M | Diagnosis | PB | 45,XY,der(1)inv(1)(p3q4)t(1;5)(?;q?),der(5)t(1;5),-7,t(9;22)(q34;q11)[10] | t(9;22)/high |
| 21 | 68.0 | F | 1st Relapse | BM | Failed‡ | NA |
| 22 | 27.0 | M | Diagnosis | BM | 46,XY,t(4;11)(q21;q23)[6]/46,XY[1] | t(4;11)/high |
| UPN . | Age (years) . | Gender . | Disease status . | Sample type . | Cytogenetics . | Major cytogenetic group/risk status . |
|---|---|---|---|---|---|---|
| 1 | 59.4 | F | Diagnosis | PB | Failed* | t(9;22)/high |
| 2 | 44.5 | F | Diagnosis | PB | 45,XX,-7,t(9;22)(q34;q11)[4] | t(9;22)/high |
| 3 | 19.5 | M | Diagnosis | BM | Normal | Normal/standard |
| 4 | 26.1 | M | Diagnosis | BM | 55-56, XY,+X,+4,+6,+11,+12,+14,+17,+18,+21,+21[cp4]/46,XY[4] | High hyperdiploidy/ standard |
| 5 | 46.4 | F | Diagnosis | BM | 46,XX,t(9;22)(q34;q11)[1]/46,idem,der(10)t(10;20)(q22;p12)?del(10)(q22q34),der(20)t(10;20)[7]/46,XX[3] | t(9;22)/high |
| 6 | 33.6 | M | Diagnosis | PB | 46,XY,+1,dic(1;9)(p1;p1)[8]/46,XY[2] | Other/standard |
| 7 | 42.6 | M | Diagnosis | PB | 46,XY,t(4;11)(q21;q23)[10] | t(4;11)/high |
| 8 | 39.7 | M | Diagnosis | BM | 46,XY,der(9)add(9)(p11)add(9)(q34)[16]/46,XY[4] | Other/standard |
| 9 | 47.7 | M | Diagnosis | BM | 46,XY[20] | Normal/standard |
| 10 | 42.8 | F | Diagnosis | PB | 46,XX,t(2;11)(p11;p11),t(4;11)(q21;q23),i(7)(q10),add(22)(q13)[18]/46,XX[2] | t(4;11)/high |
| 11 | 39.8 | M | Diagnosis | PB | 59-62,XXYY,-1,-2,-3,-7,+8,add(9)(p1),der(9)t(9;22)(q34;q11)x2,-10,-12,-14,-15,-19,-20,+21,-22,-22,+2mar[cp6] | t(9;22)/high |
| 12 | 22.7 | M | Diagnosis | BM | 47,XY,+21c[20] | t(12;21)/standard |
| 13 | 50.4 | M | Diagnosis | BM | 46,XY,t(9;22)(q34;q11)[3]/46,XY[1] | t(9;22)/high |
| 14 | 48.2 | M | Diagnosis | PB | 46,XY,t(4;11)(q21;q23)[19]/46,XY[1] | t(4;11)/high |
| 15 | 49.3 | F | Diagnosis | PB | 46,XX,del(5)(q11q1?3),add(9)(p2?4)x2,der(19)t(1;19)(q23;p13)[35]/46,XX[5] | t(1;19)/standard |
| 16 | 42.7 | M | Diagnosis | PB | 46,XY,der(15)t(1;15)(q2;p1),der(19)t(1;19)(q23;p13)[11]† | t(1;19)/standard |
| 17 | 50.2 | F | Diagnosis | BM | Normal | Normal/standard |
| 18 | 52.4 | M | Diagnosis | BM | 45∼46,XX,inv(1)(p2q2),-4,add(4)(q2),del(6)(q1q2),add(8)(q24),add(9)(p2),add(9)(q2),-11,+2mar[cp10] | Complex/high |
| 19 | 48.7 | M | Diagnosis | BM | 46,XX,t(9;22)(q34;q11.2)[16]/46,XX[4]. | t(9;22)/high |
| 20 | 32.0 | M | Diagnosis | PB | 45,XY,der(1)inv(1)(p3q4)t(1;5)(?;q?),der(5)t(1;5),-7,t(9;22)(q34;q11)[10] | t(9;22)/high |
| 21 | 68.0 | F | 1st Relapse | BM | Failed‡ | NA |
| 22 | 27.0 | M | Diagnosis | BM | 46,XY,t(4;11)(q21;q23)[6]/46,XY[1] | t(4;11)/high |
Cytogenetic profiles were determined by local laboratories using standard methods. NA, not applicable; UPN, unique patient number.
FISH positive for BCR/ABL gene rearrangement.
FISH positive for TCF3(E2A)-PBX1 gene fusion.
This case had low hypodiploidy (30-39 chromosomes) at diagnosis. FISH analysis at relapse detected loss of the ABL1 and BCR signals consistent with the low hypodiploid clone seen at diagnosis.