Table 1 Thermodynamic parameters... | American Society of Hematology

Table 1

Thermodynamic parameters (calculated per mole PF4 tetramer) for the interaction of PF4 with UFH, HO16, LMWH, HO08, HO06, and fondaparinux at 25°C

Complexes	Enthalpy, ∆H (cal/mol)	Dissociation constant, K_D (µM)	Stoichiometry, n	Gibbs free energy, ∆G (cal/mol)	Entropy, ∆S (cal/mol/K)
PF4/UFH	−6682 ± 1150	0.14	0.24 ± 0.03	−2240 ± 80	−15.6 ± 4.1
PF4/HO16	−7260 ± 1365	0.05	0.42 ± 0.1	−3865 ± 489	−11.4 ± 6.2
PF4/LMWH	−6663 ± 906	0.09	0.36 ± 0.01	−3515 ± 136	−11.5 ± 3.8
PF4/HO08	−4240 ± 1467	0.20	1.93 ± 0.42	−17 148 ± 635	46.1 ± 23.3
PF4/HO06	−2071 ± 35	2.50	0.42 ± 0.04	−3190 ± 151	3.7 ± 0.5
PF4/fondaparinux	−1333 ± 57	1.25	1.30 ± 0.15	−10 415 ± 404	30.4 ± 1.4

Complexes	Enthalpy, ∆H (cal/mol)	Dissociation constant, K_D (µM)	Stoichiometry, n	Gibbs free energy, ∆G (cal/mol)	Entropy, ∆S (cal/mol/K)
PF4/UFH	−6682 ± 1150	0.14	0.24 ± 0.03	−2240 ± 80	−15.6 ± 4.1
PF4/HO16	−7260 ± 1365	0.05	0.42 ± 0.1	−3865 ± 489	−11.4 ± 6.2
PF4/LMWH	−6663 ± 906	0.09	0.36 ± 0.01	−3515 ± 136	−11.5 ± 3.8
PF4/HO08	−4240 ± 1467	0.20	1.93 ± 0.42	−17 148 ± 635	46.1 ± 23.3
PF4/HO06	−2071 ± 35	2.50	0.42 ± 0.04	−3190 ± 151	3.7 ± 0.5
PF4/fondaparinux	−1333 ± 57	1.25	1.30 ± 0.15	−10 415 ± 404	30.4 ± 1.4

The stoichiometry n is the molar ratio heparin molecule/PF4.