The proteins in CRBN complex identified by both Co-IP and pull-down assays
| . | Co-IP . | Ni+ beads pull-down . | ||||||
|---|---|---|---|---|---|---|---|---|
| Experiment 1* . | Experiment 2* . | Experiment 1* . | Experiment 2* . | |||||
| Proteins . | NlgG . | CRBN Ab . | NlgG . | CRBN Ab . | Vector . | CRBN-His . | Vector . | CRBN-His . |
| CRBN | 0 | 3 | 0 | 9 | 0 | 32 | 0 | 35 |
| CUL4A | 0 | 2 | 1 | 8 | 0 | 34 | 1 | 31 |
| DDB1 | 7 | 123 | 9 | 47 | 11 | 122 | 3 | 82 |
| IKZF1 | 0 | 2 | 0 | 2 | 2 | 3 | 0 | 6 |
| KPNA2 | 8 | 14 | 1 | 8 | 5 | 36 | 0 | 16 |
| LTF | 2 | 5 | 1 | 4 | 3 | 16 | 1 | 33 |
| PFKL | 1 | 4 | 2 | 4 | 5 | 12 | 0 | 3 |
| PRKAR2A | 4 | 9 | 1 | 2 | 7 | 11 | 1 | 6 |
| RANGAP1 | 3 | 9 | 2 | 4 | 3 | 5 | 0 | 5 |
| SHMT2 | 10 | 25 | 3 | 8 | 4 | 8 | 1 | 4 |
| . | Co-IP . | Ni+ beads pull-down . | ||||||
|---|---|---|---|---|---|---|---|---|
| Experiment 1* . | Experiment 2* . | Experiment 1* . | Experiment 2* . | |||||
| Proteins . | NlgG . | CRBN Ab . | NlgG . | CRBN Ab . | Vector . | CRBN-His . | Vector . | CRBN-His . |
| CRBN | 0 | 3 | 0 | 9 | 0 | 32 | 0 | 35 |
| CUL4A | 0 | 2 | 1 | 8 | 0 | 34 | 1 | 31 |
| DDB1 | 7 | 123 | 9 | 47 | 11 | 122 | 3 | 82 |
| IKZF1 | 0 | 2 | 0 | 2 | 2 | 3 | 0 | 6 |
| KPNA2 | 8 | 14 | 1 | 8 | 5 | 36 | 0 | 16 |
| LTF | 2 | 5 | 1 | 4 | 3 | 16 | 1 | 33 |
| PFKL | 1 | 4 | 2 | 4 | 5 | 12 | 0 | 3 |
| PRKAR2A | 4 | 9 | 1 | 2 | 7 | 11 | 1 | 6 |
| RANGAP1 | 3 | 9 | 2 | 4 | 3 | 5 | 0 | 5 |
| SHMT2 | 10 | 25 | 3 | 8 | 4 | 8 | 1 | 4 |
The number in each column represents the number of assigned spectra for that protein.