Catalytic efficiencies of FRETS-VWF73 proteolysis by ADAMTS13 and ADAMTS13 variants
| . | WT ADAMTS13 . | Cys5 . | Gly3 . | RRYY . |
|---|---|---|---|---|
| kcat/Km (×105 M−1s−1) | 9.1 ± 1.5 | 1.5 ± 0.4 | 0.8 ± 0.1 | 1.8 ± 0.2 |
| . | WT ADAMTS13 . | Cys5 . | Gly3 . | RRYY . |
|---|---|---|---|---|
| kcat/Km (×105 M−1s−1) | 9.1 ± 1.5 | 1.5 ± 0.4 | 0.8 ± 0.1 | 1.8 ± 0.2 |
The catalytic efficiency (kcat/Km) of FRETS-VWF73 proteolysis by WT ADAMTS13 and ADAMTS13 variants Cys5, Gly3, and RRYY was derived from reactions containing 1 µM FRETS-VWF73. Reactions were performed at different enzyme concentrations for each variant and each reaction followed to completion.