Immunophenotyping of CD4+ T-cell populations
| . | Healthy (n = 28) . | Elderly (n = 6) . | SARC (n = 8) . | ICL (n = 20) . |
|---|---|---|---|---|
| % CD4+ in T CD3+ | 61.2 (44.1-71.5) | 52.8 (29.9-77.8)* | 52.3 (28.3-65.2)* | 38.7 (3.52-76.1)**,† |
| In T CD4+: | ||||
| % CCR7+ CD45RA+ (NV) | 54.9 (18.9-64.3) | 25.9 (11-37.7)*** | 41.2 (25.2-56.3)* | 27.6 (11.2-52.4)*** |
| % CCR7+ CD45RA− (CM) | 28.2 (12.3-41.4) | 19.7 (9.5-49.7) | 10.2 (5.2-29.1)** | 18.2 (5.1-41.3) |
| % CCR7− CD45RA− (EM) | 15.6 (5.2-35.5) | 23.3 (14.6-37.5)* | 20.4 (15.3-47.3)* | 30.4 (10.1-44.9)** |
| % CCR7− CD45RA+ (TEMRA) | 10.1 (5-22.3) | 31 (17.2-42.5)* | 19.6 (17.3-30.2)* | 28.5 (12.5-40.8)** |
| Correlation factor (r Spearman’s and P value) | ||||
| Percentage naive and CD4+ T-cell count | ns | 0.90 and 0.04 | ns | 0.44 and 0.049 |
| Percentage activated/memory and CD4+ T-cell count | ns | −0.90 and 0.04 | ns | −0.54 and 0.014 |
| CXCR4 in T CD4+(MFI) | ||||
| Total | 586 (189-805) | 572 (190-1231) | 500 (248-610) | 199 (47-639)***,† |
| NV | 912 (610-1010) | 927 (712-1425) | 897 (745-958) | 287 (112-314)***,† |
| CM | 294 (112-357) | 274 (97-412) | 257 (100-387) | 124 (58-220)*,† |
| EM | 151 (92-245) | 167 (102-258) | 102 (56-257) | 64 (42-112)**,† |
| TEMRA | 382 (212-478) | 427 (247-528) | 450 (312-524) | 77 (55-97)***,† |
| CXCR4 in T CD4+(%) | ||||
| Total | 61.5 (38.4-75.7) | 67 (37.2-89.3) | 60.4 (42.1-76.2) | 39.5 (12.4-65.7)**,† |
| NV | 80.2 (64.1-94-1) | 84.3 (68.2-97.4) | 77.5 (55.2-84.3) | 39.1 (12.6-48.4)***,† |
| CM | 53.2 (44.7-67.7) | 56.1 (45.3-71.3) | 49.7 (32.1-55.4) | 32.4 (22.8-48.6)*,† |
| EM | 40.7 (35.1-52.1) | 39.3 (42.1-64.8) | 42.4 (22.4-58.4) | 24.9 (11.3-28.7)**,† |
| TEMRA | 59.5 (32.4-64.4) | 66.2 (42.1-77.3) | 51.8 (33.7-64.7) | 26.4 (8.3-32.7)**,† |
| Correlation factor (r Spearman’s and P value) | ||||
| CXCR4 MFI and CD4+ T-cell count | ns | ns | ns | 0.45 and 0.039 |
| CXCR4 % and CD4+ T-cell count | ns | ns | ns | 0.46 and 0.035 |
| . | Healthy (n = 28) . | Elderly (n = 6) . | SARC (n = 8) . | ICL (n = 20) . |
|---|---|---|---|---|
| % CD4+ in T CD3+ | 61.2 (44.1-71.5) | 52.8 (29.9-77.8)* | 52.3 (28.3-65.2)* | 38.7 (3.52-76.1)**,† |
| In T CD4+: | ||||
| % CCR7+ CD45RA+ (NV) | 54.9 (18.9-64.3) | 25.9 (11-37.7)*** | 41.2 (25.2-56.3)* | 27.6 (11.2-52.4)*** |
| % CCR7+ CD45RA− (CM) | 28.2 (12.3-41.4) | 19.7 (9.5-49.7) | 10.2 (5.2-29.1)** | 18.2 (5.1-41.3) |
| % CCR7− CD45RA− (EM) | 15.6 (5.2-35.5) | 23.3 (14.6-37.5)* | 20.4 (15.3-47.3)* | 30.4 (10.1-44.9)** |
| % CCR7− CD45RA+ (TEMRA) | 10.1 (5-22.3) | 31 (17.2-42.5)* | 19.6 (17.3-30.2)* | 28.5 (12.5-40.8)** |
| Correlation factor (r Spearman’s and P value) | ||||
| Percentage naive and CD4+ T-cell count | ns | 0.90 and 0.04 | ns | 0.44 and 0.049 |
| Percentage activated/memory and CD4+ T-cell count | ns | −0.90 and 0.04 | ns | −0.54 and 0.014 |
| CXCR4 in T CD4+(MFI) | ||||
| Total | 586 (189-805) | 572 (190-1231) | 500 (248-610) | 199 (47-639)***,† |
| NV | 912 (610-1010) | 927 (712-1425) | 897 (745-958) | 287 (112-314)***,† |
| CM | 294 (112-357) | 274 (97-412) | 257 (100-387) | 124 (58-220)*,† |
| EM | 151 (92-245) | 167 (102-258) | 102 (56-257) | 64 (42-112)**,† |
| TEMRA | 382 (212-478) | 427 (247-528) | 450 (312-524) | 77 (55-97)***,† |
| CXCR4 in T CD4+(%) | ||||
| Total | 61.5 (38.4-75.7) | 67 (37.2-89.3) | 60.4 (42.1-76.2) | 39.5 (12.4-65.7)**,† |
| NV | 80.2 (64.1-94-1) | 84.3 (68.2-97.4) | 77.5 (55.2-84.3) | 39.1 (12.6-48.4)***,† |
| CM | 53.2 (44.7-67.7) | 56.1 (45.3-71.3) | 49.7 (32.1-55.4) | 32.4 (22.8-48.6)*,† |
| EM | 40.7 (35.1-52.1) | 39.3 (42.1-64.8) | 42.4 (22.4-58.4) | 24.9 (11.3-28.7)**,† |
| TEMRA | 59.5 (32.4-64.4) | 66.2 (42.1-77.3) | 51.8 (33.7-64.7) | 26.4 (8.3-32.7)**,† |
| Correlation factor (r Spearman’s and P value) | ||||
| CXCR4 MFI and CD4+ T-cell count | ns | ns | ns | 0.45 and 0.039 |
| CXCR4 % and CD4+ T-cell count | ns | ns | ns | 0.46 and 0.035 |
Data are medians (ranges). Using the protein tyrosine phosphatase isoform CD45RA and the chemokine receptor CCR7, CD4+ T cells were subdivided into naive (NV), central memory (CM), effector memory (EM), and terminally differentiated effector memory (TEMRA) subpopulations. All P values are compared with healthy individuals.
ns, not significant.
P < .05.
P < .005.
P < .0005.
Significantly different compared with elderly subjects (as determined using the Mann-Whitney U test).