Affinity and CDR3 sequences of the parental Fab 72 and optimized variants
| Antibody . | VH CDR3 . | VL CDR3 . | Hapten . | Equilibrium, KD . | 95% Confidence interval . |
|---|---|---|---|---|---|
| Fab 72 | GSHLY99DFW100bSASHPPNDALAI | GTW91D92I93S94LSAGL | Ticagrelor | 7.4 nM | 1.8-21.5 nM |
| Fab 152 | GSHLYDFWSASHPPNDALAI | GTWLYDRAVGL | Ticagrelor | 43.2 pM | 2.8-119.2 pM |
| Fab 162 | GSFDYYFWSASHPPNDALAI | GTWDISLSAGL | Ticagrelor | 162.5 pM | 125.4-206.3 pM |
| MEDI2452 | GSFDYYFWSASHPPNDALAI | GTWLYDRAVGL | Ticagrelor | 19.6 pM | 13.0-28.7 pM |
| TAM | 19.7 pM | 4.9-44.7 pM | |||
| TIM | ∼20 nM |
| Antibody . | VH CDR3 . | VL CDR3 . | Hapten . | Equilibrium, KD . | 95% Confidence interval . |
|---|---|---|---|---|---|
| Fab 72 | GSHLY99DFW100bSASHPPNDALAI | GTW91D92I93S94LSAGL | Ticagrelor | 7.4 nM | 1.8-21.5 nM |
| Fab 152 | GSHLYDFWSASHPPNDALAI | GTWLYDRAVGL | Ticagrelor | 43.2 pM | 2.8-119.2 pM |
| Fab 162 | GSFDYYFWSASHPPNDALAI | GTWDISLSAGL | Ticagrelor | 162.5 pM | 125.4-206.3 pM |
| MEDI2452 | GSFDYYFWSASHPPNDALAI | GTWLYDRAVGL | Ticagrelor | 19.6 pM | 13.0-28.7 pM |
| TAM | 19.7 pM | 4.9-44.7 pM | |||
| TIM | ∼20 nM |
Changes in sequences from Fab 72 are in bold type. Key residues relating to Figure 2 are followed by Kabat numbers in superscript.